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1999 Fiscal Year Final Research Report Summary

Establishment of typing system of MRSA by arbitrarily-primed PCR

Research Project

Project/Area Number 09670617
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionKagawa Medical university

Principal Investigator

FUJITA Jiro  Kagawa Medical University, 1st Dept Int Med, Research Associate, 医学部, 助手 (80209056)

Co-Investigator(Kenkyū-buntansha) OKADA Hiroki  Kagawa Medical University, University Hospital, Lecturer, 医学部・附属病院, 講師 (00243775)
Project Period (FY) 1997 – 1999
KeywordsArbitrarily-primed PCR / MRSA / Typing / Nosocomial infection
Research Abstract

In 1997, we established the typing method for methicillin-resistant Staphylococcus aureus (MRSA) by an arbitrarily-primed polymerase. Chain reaction. In 1998, we applied this method to several strains of MRSA isolated in Kagawa Medical University Hospital. Usefulness of the typing method by AP-PCR was compared to other typing methods: phenotypic characteristics (production of enterotoxin and TSST-1, antimicrobial susceptibity) and molecular typing procedure (plasmid DNA profile, pulsed-field gel electrophoresis [PFGE] . Although traditional epidemiological methods for example, coagulase typing plays a central role in hospital infection control, combination of plasmid DNA profile, AP-PCR, and PFGE may prove to be a particularly informative means of tracking the nosocomial spread of MRSA. Using AP-PCR, we could prove the nosocomial infection caused by cagulase VII positive MRSA strain .
In 1999, we applied this method to other bacteria : Pseudomonas cepacia, Pasteurella multocida, and Mycobacterium avium intracellulare complex, and we demonstrated that AP-PCR could be a useful and simple method to investigate the transmission of several bacteria.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yuka Obayash: "Investigation of rnosocomial infection due to arbekacln-resistant methicillin-reslstant Staphylococcus aureus"Diag Microbiol Infect Dis. 28. 53-59 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jiro Fujita: "Pneumcnla caused by Pseudomonas putida with a mucoid phenotype"Respir Med. 92. 693-695 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Miyawaki: "Detection of Pneumocystis carinii squences in serum by polymerase chain reaction:clinicial application in two patients with Pneumocystis carinii pneumonia"J Infect Chemother. 4. 36-39 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoko Hojo: "Severe cystic flbrosis associated with a △F508/R347H+D1D979A compound heterozygous genotype"Clin Genetics. 53. 50-53 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuka Obayash: "Clinical features of non-tuberculous mycobacterium.-Comparison between smear-positive cases and culture-positive cases,as well as comparison between Mycobacterium avium and Mycobavterium intracellular-"Int J Tuberc Lung Dis. 2. 597-602 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jiro Fujita: "Pathological and Immunohistochemical findings of resected lung specimens obtained from patients with Mycobacterium avium intracellular complex"Eur Respir J. 13. 535-540 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuka Obayash: "Successive follow-up of chest computed tomography in patietns with Mycobacterium avium-intracellulare complex"Respir Med. 93. 11-15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤田次郎: "耐性菌感染症の理論と実際(平松啓一編)"ハベカシン耐性、コアグラーゼVII型MRSAによる院内感染症. 4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Obayashi. J. Fujita, S. Hojo, K. Negayama, H. Miyawaki, Y. Yamaji, K. Kawanishi, J. Takahara.: "Investigation of nosocomial infection due to arbekacin-resistant methicillin-resistant Staphylococcus aureus."Diag Microbiol Infect Dis. 28. 53-59 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Fujita, K. Negayama, M. Ohara, S. Hojo, Y. Obayashi, H. Miyawaki, Y. Yamaji, J. Takahara: "pneumonia caused by Pseudomonas putida with a mucoid phenotype."Respir Med. 92. 693-695 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Miyawaki, J. Fujita. I. Yamadori, S. Hojo, Y. Obayashi, H. Nakamura, Y. Yamaji, l. Suemitsu, M. Harada, S. Suguri, J. Takahara: "Detection of Pneumocystis carinii sequences in serum by polymerase chain reaction : clinical application in two patients with Pneumocystis carinii pneumonia."J Infect Chemother. 4. 36-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Hojo, J. Fujita, H. Miyawaki, Y. Obayashi, J. Takahara, D. W. Bartholomew: "Severe cystic fibrosis associated with a ΔF508/R347H + D979A compound heterozygous genotype."Clin Genetics. 53. 50-53 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Obayashi, J. Fujita, I. Suemitsu, T. Kamei, M. Nii, J. Takahara: "Clinical features of non-tuberculous mycobacterium. -Comparison between smear-positive cases and culture-positive cases, as well as comparison between Mycobacterium avium and Mycobacterium intracellulare-."Int J Tuberc Lung Dis. 2. 597-602 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J. Fujita, Y. Ohtsuki, I. Suemitsu, I. Yamadori, E. Shigetou, Y. Obayashi, H. Miyawaki, N. Dobashi, T. Matsushima, J. Takahara: "Pathological and immunohistochemical findings of resected lung specimens obtained from patients with Mycobacterium avium intracellulare complex."Eur Respir J. 13. 535-540 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Obayashi, J. Fujita, I. Suemitsu, T. Kamei, M. Nii, J. Takahara: "Successive follow-up of chest computed tomography in patietns with Mycobacterium avium-intracellulare complex."Respir Med. 93. 11-15 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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