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1998 Fiscal Year Final Research Report Summary

The role of nitric oxide in PGI_2 production by human pulmonary artery smooth muscle cells

Research Project

Project/Area Number 09670636
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionFukuoka University

Principal Investigator

WATANABE Kentaro  Fukuoka Univ., School of Med., Assist.Prof., 医学部, 講師 (80158625)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Minoru  Fukuoka Univ., School of Med., Professor, 医学部, 教授 (60078772)
Project Period (FY) 1997 – 1998
Keywordshuman pulmonary artery smooth muscle cells / prostacyclin(PGI_2) / nitric oxide(NO,nitrogen monooxide) / cytokines / lipopolysaccharide(LPS) / IL-6
Research Abstract

Human pulmonary arlcry smooth musclc cclls(HPASMC) were isolated and culfured from autopsy materials, and the effect of nitric oxide(NO) on PG12 production by HPASMC was examined and the effect of IL-6 on NO production by HPASMC was also examined to elucidate the relationship of the production of NO and proslaglandins by H PASMC.
1)LPS, IL-1beta and TNF_2 enhanced the production of NO by HPASMC.
2)The enlhanced production of PGI_2 by LPS-and IL-1beta-treated HPASMC was further augmenter when HPASMC were treated with LPS or IL-1beta together with sodium nitroprusside.
3)The enhanced production of PGI_2 by LPS- and IL-1beta-treated HPASMC was attenuated when HPASMC were treated with LPS or IL-1beta together with L-NMMA.
4)Methylene blue(MeB) suppressed PGI_2 production by HPASM C, and attenuated the enhanced production of PGI_2 by HPASMC treated with LPS or IL-1beta.
5)Although IL-6 did not changed the basal production of NO by HPASMC, it attenuated the enhanced production of NO by HPASMC treated with LPS or IL-1beta.
6)LPS, IL-1beta, TNF_2 and SNP up to the maximum concentrations used in these experiments did not increase thc LDH release from HPASMC treated with these agents.
From these experimental lindings it is suggested that
(1)NO enhances PGI_2 production by human pulmonary arlery smooth muscle cells.
(2)IL-6 suppresses not only the production of prostagland ins but NO by human pulmonary artery smooth muscle cells. this result could show antiinllammatory aspect of IL-6 in vivo.
(3)Pulmonary artery smooth lb muscles could actively participate in the regulation of pumonary blood flow and tonus of pulmonary artery by the interaction of NO and prostaglandins.
Further examinations are needed to elucidate the. mechanisms of NO-mediated enhancement of PGl_2 production and IL-6-mediated suppression of NO production by examining the induction of COX-2 and iNOS proteins and genes.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] K Watanabe, FQ Wen, M Yoshida: "Nitric oxide stimulates PGI_2 production by human pulmonary artery smooth muscle cells" Proceedings of Pulmonary Circulation Research. 13-18 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] FQ Wen, K Watanabe, M Yoshida: "Eicosanoid profile in cultured human pulmonary artery smooth muscle cells treated with IL-1β and TNFα" Prostaglandins Leuukotrienes and Essential Fatty Acids. 59(1). 71-75 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] FQ Wen, K Watanabe, M Yoshida: "Lipopolysaccharide enhances the release of arachidonic acid metabolites in cultured human pulmonary artery smooth muscle cells" Artery, in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺憲太郎, 文 富強, 信国寿美重, 吉田 稔, 他: "NOはサイトカインやエンドトキシンによるヒト肺動脈平滑筋細胞のPGI_2産生を促進する" Therapeutic Research. 19(4). 1159-1164 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺憲太郎: "血管内皮細胞・平滑筋のプロスタグランディン代謝" Progress in Medicine. 19(1). 222-232 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺憲太郎: "肺血管内皮細胞の培養と呼吸器疾患" 医学のあゆみ 呼吸器疾患-state of arts Ver.3. 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kentaro Watanabe, Fu-Qiang Wen, Minoru Yoshida: "Nitric oxide stimulates PGI_2 production by human pulmonary artery smooth muscle cells." Proceedings Pulmonary Circulation Research. 13-18 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fu-Qiang Wen, Kentaro Watanabe, Minoru Yoshida: "Eicosanoid profile in cultured human pulmonary artery smooth muscle cells treated with IL-1beta and TNFalpha" Prostaglandins, Leukotrienes and Essential Fatty Acids. 59. 71-75 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fu-Qiang Wen, Kentaro Watanabe, Minoru Yoshida: "Lipopolysaccharide enhances the release of arachidonic acid metabolites in cultured human pulmonary artery smooth muscle cells." Artery. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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