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1998 Fiscal Year Final Research Report Summary

Localization of glial-cell line derived neurotrophic factor receptor (GFRa1) in the brain

Research Project

Project/Area Number 09670654
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAKAMURA Shinichi  Kyoto University Neurology Instructor, 医学研究科, 助手 (20231475)

Co-Investigator(Kenkyū-buntansha) AKIGUCHI Ichiro  Kyoto University Neurology Associate Professor, 医学研究科, 助教授 (30115779)
Project Period (FY) 1997 – 1998
KeywordsGlial cell line-derived neurotrophic factor / GDNF / GDNF-receptor / GDNFR / immunohistochemistry / rat brain
Research Abstract

Glial cell line-derived neurotrophic factor (GDNFR) has been shown to have trophic effect upon dopaminergic neurons and motoneurons in the anterior horn cells of spinal cord. In the present research project we have planned to localize GDNFR in the brain immunohistochemically and examine whether GDNFR is involved in the pathophysiology of neurodegenerative disease such as Parkinson's disease, amyotrophic lateral sclerosis, multiple system atrophy and Alzheimer's disease.
We have firstly developed an anti-peptide antibody against synthetic peptide of amino-terminal portion of GDNFR The antibody recognized a single protein of molecular weight of 80 kDa in the rat brain homogenate. The preabsorption of the antibody with the immunogen completely abolished the immunostaining in the western blot lane. The immunohistochemical study has demonstrated GDNFR-immunoreactivity exclusively in neurons and no glial cells were immunopositive. The immunopositive neuronal somata were found throughout the rat brain. In the neocortex the positive neurons were distributed in the layer II through to layer VI.Pyramidal and non-pyramidal cell were both labeled. In the basal forebrain, numerous large sized neurons were immunolabeled in the nucleus of Meynert, medial septal nucleus, nucleus, of diagonal band of Broca and ventral pallidum. Numerous neuronal somata were found also in nuclei of the hind brain, including substantia nigra, raphe, and cochlear nucleus. Thus, GDNF responsive neurons appear to be distributed more widespread in the rat brain than previously described.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kawamoto Y.et al.: "Cellular localization of brain-derived neurotrophic factor-like immunoreactivity in adult monkey brain" Brain Res.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto Y.et al.: "Increased brain-derived neurotrophic factor-containg axons in the basal ganglia of patients with multiple system atrophy" J.Neuropathol.Exp.Neurol.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura S.et al.: "Cyclin-dependent kinase 5 and mitogen-activated protein kinase in glial cytoplasmic inclusions in multiple system atrophy" J.Neuropathol.Exp.Neurol.57. 690-698 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto Y.et al.: "Immunohistochemical localization of brain derived neurotrophic factor in the spinal cords of amyotrophic lateral sclerosis and non-amyotrophic lateral sclerosis patients" J.Neuropathol.Exp.Neurol.57. 822-830 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura S.et al.: "p35nck5a and cyclin-dependent kinase 5 colocalize in Lewy bodies of patients with Parkinson's disease" Acta Neuropathologica. 94. 153-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura S.et al.: "Cyclin-dependent kinase 5 in Lewy body-like inclusion in anterior horn cells of a patient with sporadic amyotrohic lateral sclerosis" Neurology. 48. 267-270 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto Y et al.: "Cellular localization of brain derived neurotrophic factor-like immunoreactivity in adult monkey brain." Brain Res.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto Y.et al.: "Increased brain0derived neurotrophic factor-contaiing axons in the basal ganglia of patients with multiple system atrophy" J.Neuropathol.Exp.Neurol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura S.et al.: "Cyclin-dependent kinase 5 and mitogen-activated protein kinase in glial cytoplasmic inclusions in multiple system atrophy" J.Neuropathol.Exp.Neurol.57. 690-698 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto Y.et al: "Immunohistochemical localization of brain-derived neurotrophic factor in the spinal cords of amyotrophic lateral sclerosis and non-amyotrophic lateral sclerosis patients" J.Neuropathol.Exp.Neurol.57. 822-830 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura S.et al.: "p35nck5a and cyclin-dependent kinase 5 colocalize in Lewy bodies of patients with Parkinson's disease" Acta Neuropathologica. 94. 153-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura S.et al.: "Cyclin-dependent kinase 5 in Lewy body-like inclusion in anterior horn cells of a patient with sporadic amyotrohic lateral sclerosis" Neurology. 48. 267-270 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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