1998 Fiscal Year Final Research Report Summary
Apoptosis in neuronal cell death of neurodegenerative disorders.
| Project/Area Number |
09670668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | JUNTENDO UNIVERSITY |
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Principal Investigator |
MORI Hideo Juntendo Univ.Dept.of Neurol Assistant Professor, 医学部, 講師 (30150634)
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| Co-Investigator(Kenkyū-buntansha) |
SUGITA Yukihiro Juntendo Univ.Dept.of Neurol Assistant Professor, 医学部, 講師 (70162881)
MIZUNO Yoshikuni Juntendo Univ.Dept.of Neurol Professor, 医学部, 教授 (30049043)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Parkinson disease / Neuronal cell death / Apoptosis / Bcl-2 / Bcl-xL |
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| Research Abstract |
The cause and mechanism of neuronal cell death in the substantia nigra of patients with Parkinson disease (PD) is unknown and there is controversy whether apoptosis play a role in the neuronal death in PD.We investigated apoptotic-like body in the substantia nigra of PD, and progressive supranuclear palsy (PSP) using In situ end labeling method on the substantia nigra of the postmortem brain. Apoptotic-like bodies were seen in the brains of the control , PD and PSP.There were no correlation between the number of apoptotic-like bodies and age at death or the postmortem time. The mean number of apoptotic-like bodies were not significantly different between control, PD and PSP.In addition, the inimunohistochemistry against ss-DNA, which are thought as early marker of the apoptosis, showed no significant difference between PD and control in the number of ss-DNA positive neurons.
However, the immunohistochemistry for the Bcl-2 family showed the proportion of Bcl-xL-positive neurons was significantly lower in PD than control, although the proportions of Bcl-2 and Bax-positive neurons were not different between PD and control. Reduced expression of Bcl-xL may play a role in the vulnerability of the neurons in the substantia nigra of PD.
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