1999 Fiscal Year Final Research Report Summary
Coronary angiogenesis induced by VEGF gene: Comparison of angiogenic vessels and gene expression
| Project/Area Number |
09670756
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Tokai University |
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Principal Investigator |
MORI Hidezo Tokai University School of Medicine, Associate Professor, 医学部, 助教授 (90146598)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Sumihisa Tokai University School of Medicine, Assistant Professor, 医学部, 講師 (50159430)
ANDO Kiyoshi Tokai University School of Medicine, Assistant Professor, 医学部, 講師 (70176014)
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| Project Period (FY) |
1997 – 1999
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| Keywords | VEGF / ASO / angiogenesis / synchrotron radiation / angiography / microsphere / adeno-virus |
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| Research Abstract |
In this term of the project, we revealed that Lac-Z could be introduced into ischemic myocardial tissue by using adeno-virus vector, that VEGF165 containing adeno-virus relieved the ischemic myocardium and that new drug delivery system: gelatin hydrogel enhanced the efficiency of gene therapy without using adenovirus vector. In H-9, microvascular ischemia was created in 30 rabbits. One hour after the ischemia, gene delivery was performed. Four weeks later, changes in myocardial function and myocardial blood flow were compared with those in the acute pahse of ischemia. In H-10, we tested whether efficiency of gene (VEGF) delivery can be enhanced by the use of gelatin hydrogel in rabbit ASO model. In H-11, supplemental experiments were added and scientific manuscript was written.
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