| Co-Investigator(Kenkyū-buntansha) |
DEGUCHI Hirofumi Faculty of Medicine, Osaka Medical College, Assistant Professor, 医学部, 助教授 (90131341)
KITAURA Yasushi Faculty of Medicine, Osaka Medical College, Professor, 医学部, 教授 (50084950)
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| Research Abstract |
In order to detect the localization of viral genomes in the myocardium, we examined murine hearts with Coxsackievirus B3(CVB3) myocarditis by in situ hybridization. CVB3-RNA was detected in the cytoplasm of cardiocytes, between the myofibrils, near the mitochondria, and in tubular or vesicular structures. Viral RNA was also detected in necrotic debris, in the cytoplasm of macrophages, and in the cytoplasm of interstitial fibroblasts. These findings suggest that CVB3-RNA is replicated in the cytoplasm of cardiocytes, transferred into tubular or vesicular structures, released into the interstitium, and phagocytosed by macrophages. Some positive signals were also detected in the cytoplasm of cardiocytes showing close contact with infiltrating lymphocytes, suggesting that the lymphcytes recognized virus-infected cardiocytes and caused to cell-mediated immune cardiocyte damage.
We detected TUNEL-positive myocyte and DNA fragmentation by gel electrophoresis of extracted DNA in murine viral myocarditic hearts. However, we could not detect typical apoptotic myocyte with the condensation of chromatin in murine viral myocarditic hearts. We analyzed viral genome by PCR and quantitative detection of Mrna of Fas, Bcl-2 and Bax in the resected myocardium of DCM patients. Enteroviral RNA was detected in eight of the 24 patients of DCM. We detected TTUNEL-positive myocyte, however the expression of ischemic heart disease patients. It is not considered that apoptotic processes is important in DCM or viral chronic myocarditis.
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