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1998 Fiscal Year Final Research Report Summary

Teratologic effects of Bis-diamine on cardiovascular development in rat embryo : in vivo and in vitro analysis

Research Project

Project/Area Number 09670800
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionShiga University of Medical Science

Principal Investigator

NAKAGAWA Masao  Shiga University of Medical Science Dept.of Pediatrics, Associate Professor, 医学部, 講師 (40188909)

Project Period (FY) 1997 – 1998
Keywordsconfocal laser scanning micro-scope / aortic arch anomaly / bis-diamine / whole embryo culture / elongated outflow tract / pericardial defect / enlarged ventricle / delayed neural tube closure
Research Abstract

Bis-diamine administration to pregnant rats induces conotruncal anomalies.
To know teratologic effects of bis-diamine depending on the developmental stage, we examined neonates and embryos from Wister mother rats to which a single dose of 200 mg his-diamine was administered at 10, 11, or 12 days of gestation (group 1, 2, and 3, respectively). The neonates from group 1 (n 86) had aortjc arch anomalies in 100 %, and those from group 2 (n = 95) and 3 (n = 108) had aortic arch anomalies and truncus arteriosus communis in 92.8%, and aortic arch anomalies and tetralogy of Fallot in 45.4%, respectively. In order to examine the early morphological effects of bis-diamine, we cultured four embryos from each group for 24 hours following the procedures described by Cockroft and New (1978). The embryos from group 1 showed delayed closure of the neural tube and immature cardiac looping with undifferentiated third, fourth, and sixth Nortek arches. Immunohistological study using anti-N-CAM antibody revealed that N-CAM positive cells were sparsely distributed around the neural tube. The embryos from group 2 had an enlarged and dilated ventricle and winding and elongate outflow tract in which regurgitant blood flow was observed, whereas none in controls. Furthermore, two of the embryos had a partial pericardial defect. A continuous N-CAM immunopositive chain from the neural tube to the heart was clearly seen, though thin when compared with controls. No morphological and circulatory difference was seen between embryos from group 3 and controls.
These results suggested that bis-diamine may disturb normal development and migration of cardiac neural crest or mesenchymal cells to induce various cardiovascular anomalies depending on the developmental stage.

  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Masao Nakagawa: "Analysis of heart development in cultured rat embryos." J Mol Cell Cardiol. 29.2. 369-379 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akemi Matsui: "Association of atrial septal defect with Poland-Moebius syndrome." Angiology. 48.3. 269-271 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masashi Takahashi: "Horseshoe lung : demonstration by electron-beam CT." Brit J Radiol. 70.964-966 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Teebi hyperterolism syndrome with tetralogy of Fallot." Am J Med Genet. 77.5. 345-347 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Intractable diarroea with serious tetany in "CATCH 22"." J Pediatr Gastr Nutr. 27.2. 222-223 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Ebstein's anomaly associated with trisomy 9p." Clin Genet. In Press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Detection and evaluation of asymptomatic myocarditis in school children : report of four cases." Chest. In press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akemi Sato: "Thrombocytopenia after human herpes virus-7 infection in a patient with DiGeorge syndrome." J Pediatr Hematol/Oncol. 21.2In press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 奥野昌彦: "ウイルス性急性心筋炎における心筋細胞のDNA合成について-BrdUflashのlabeling法を用いた経時的検討-" 心筋の構造と代謝-1996-. 19. 239-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 成田 努: "大動脈弁閉鎖不全を合併した左単冠動脈の一症例" 日小循誌. 13.4. 540-543 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡川浩人: "川崎病のQT時間について" Prog Med. 17. 1761-1764 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 入江直子: "学校心電図検診にて発見された右室異形成症の1例" 心臓. 29.9. 777-782 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岡川浩人: "川崎病冠動脈瘤に合併した狭窄性病変に対しDirectional Coronary Atherectomy(DCA)により狭窄部解除を試みた1症例" 小児科診療. 61. 1347-1352 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松井明美: "学校心電図検診で発見された無症候性心筋炎の1例" 小児科臨床. 51. 210-214 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤野英俊: "ファロー四徴症児の発達" 小児保健研究. 57.4. 576-580 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Etiology and Morphogenesis of Congenital Heart Disease" Futura Publishing Co. In press, (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中川雅生: "小児科の進歩 17" 前川喜平、今村栄一編、診断と治療社, 255 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masao Nakagawa: "Analysis of heart development in cultured rat embryos." J Mol Cell Cardiol. 29. 369-379 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akemi Matsui: "Association of atrial septal defect with Poland-Moebius syndrome : vascular disruption can be a common etiologic factor." Angiology. 48. 269-271 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masashi Takahashi: "Horseshoe lung : demonstration by electron-beam CT." Brit J Radiol. 70. 964-966 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Nakagawa: "Teebi hypertelorism syndrome with tetralogy of Fallot." Am J Med Genet. 77. 345-347 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Nakagawa: "Intractable diarrhoea with serious tetany in "CATCH 22"" J Pediatr Gastr Nutr. 27. 222-223 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Nakagawa: "Ebstein's anomaly associated with trisomy 9p." Clin Genet. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akemi Sato: "Thrombocytopenia after human Herpesvirus-7 infection in a patient with DiGeorge syndrome." J Pediatr Hematol/Oncol. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Nakagawa: "Detection and evaluation of asymptomatic myocarditis in schoolchildren ; report of four cases" Chest. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masao Nakagawa: "Effects of bis-diamine on cardiovascular development in an early rat embryo : in vivo and in vitro morphological analysis." Etiology and Morphogenesis of Congenital Heart Disease, Futura, New York. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsutomu Narita: "A case of single coronary artery associated with aortic regurgitation" Acta Cardiol Pediatr Jpn. 13 (In Japanese). 540-543 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiko Okuno: Cardiomyocytic DNA synthesis in viral acute myocarditis-flash labeling analysis using BrdU-. Structure and Metabolism of the Heart, 19 Roppou Publishing Co (In japanese), 239-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidetoshi Fujino: Development in a child with tetralogy of Fallot.J Child Health 57 (In Japanese), 576-580 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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