1998 Fiscal Year Final Research Report Summary
Study on cytological characteristics of myelodysplastic syndrome (MDS) in children.
| Project/Area Number |
09670830
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | DOKKYO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
EGUCHI Mitsuoki Dokkyo University School of Medicine, Medicine, Professor, 医学部, 教授 (60020799)
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| Project Period (FY) |
1997 – 1998
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| Keywords | myelodysplastic syndrome / electron microscope / peroxidase / megakryoblasts / Down's syndrome / Fanconi's anemia / blast cell / apoptosis |
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| Research Abstract |
Erytliroblast, granulocyte and megakaryocyte series of cells from MDS in children and adults were studied by electron microscopic and cytochemical methods. Fourty three patients of child, adult and secondary cases of MDS were included in this study. Major abnormal finding of granulocytes was nuclear pocket, which was most frequently observed in the cells from patients with Down's syndrome (4 out of 7 cases). Other abnormal findings in granulocytes were deformation of nucleus, Auer body-like elongated granules, and Chediak-Higashi-like large granules. In erythroblasts, sponge-like deformation of nuclear chromatin was predominant in children. Polynudear and deformed nuclear erytliroblasts were also noted. Megakaryocyte and platelet changes, such as microinegakaryocytes and giant platelets, 'were not frequently observed. Refractory anemia revealed predominant erythroblastic changes, that is, iron-loaded mitochondda was observed in 5 out of 11 patients, and sponge-like change of nucleus in 4 out of 11 cases. RAEB-T showed most predominant granulocytic changes. Nuclear pockets were obseved in 8 out of 11 cases in this sub-type of MDS.Apoptosis was observed in erythroblast and granulocyte series of cells in all MDS sub-types.
Cytochemically, the number of myeloperoxidase-posilive granules were decreased in MIDS.Some of immature cells in MDS showed platelet peroxidase reaction, suggesting that the blasts had megakaryocyte characteristics. Electron microscopic and cytochemical methods were useful to find abnormalities in MDS cells which could not detect by lightmicroscopic techniques, and MiDS of children was not identical to that of adults.
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