1998 Fiscal Year Final Research Report Summary
The research on the offect of the IGF-II/IGF-II receptor system on the infant growth.
| Project/Area Number |
09670831
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Saitama medical School |
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Principal Investigator |
SASAKI Nozomu Saitama medical School, school of Medicine, professor, 医学部, 教授 (00009672)
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| Co-Investigator(Kenkyū-buntansha) |
OHTAKE akira Saitama medical School, School of Medicine, Assistant Professor, 医学部, 講師 (00203810)
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| Project Period (FY) |
1997 – 1998
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| Keywords | INSULIN-LIKE GROWTH FACTOR II / INFANT GROWTH DISORDERS / MOLECULAR BIOLOGY / ANTI-OLIGOPEPTIDE ANTIBODY |
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| Research Abstract |
It was made that this study produced the antibody for the human IGF-II receptor and that it makes the assay system to be a goal. Results are as follows.
1. The purification from the placenta of the human IGF-II receptor : Though it succeeded, the yield point is 3.3 μg/g tissue. 2. Expression and purification in a Escherichia coli of the human IGF-II receptor : The extracellular part of the IGF-II receptor was divided into five, each cDNA was included in the expression vector. Though the expression was confirmed on third part ( the 951-1384 residue ) and fifth part ( the 1821-2304 residue ), the confirmation was not possible on the other part. 3. Peptide production of antibody for the human IGF-II receptor : 3 kinds of peptides of the human IGF-II receptor of 7-22 residue, 1103-1119 residue, 2046-2064 residue were chosen as an antigen. The carrier protein was combined, and the antibody for all peptides was got. At present, IgG fraction of the rabbit serum is being refined. The construction of assay system of the human IGF-II receptor is tried after the purification. The development of sandwich process and competition method which utilizes the mini IGF-II receptor as a standard is considered. It is parallel to the development of the assay system, in the element of the informed consent, blood and liquor amnii of the pregnant woman, sampling of IUGR children, low statue infants, blood of the overgrowth child, etc. besides have been started.
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[Publications] Kanazawa, M., Yano, M., Namchai C., Yamamoto S., Ohtake A., Takayanagi M., Mori, M. & Niimi, H.: "Visualization of mitochondria with green fluorescent protein in cultured fibroblasts from patients with mitochondrial diseases"Biochem. Biophys. Res. Commum. 239(2). 580-584 (1997)
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[Publications] Kohgo, T., Satoh, Y., Kanazawa, M., Yamamoto, S., Takayanagi, M., Ohtake, A., Mori, M. & Niimi, H.: "Expression analysis of two mutant human ornithine transcarbamylases in Cos-7 cells"J. Hum. Genet. 43(1). 54-58 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Kaneda, M., Sakuraba, H., Ohtake, A., Nishida, A., Kiryu, C. & Kakinuma, K.: "Missense mutations in the gp91-phox gene encoding cytochrome b558 in patients with cytochrome b positive and negative X-linked chronic granulomatous disease"Blood. 93(6). 2098-2104 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Nagasaka, H., Kobayashi, K., Yorifuji, T., Kage, M., Kimura, A., Takayanagi, M., Kikuta, H., Egawa, H., Tanaka. K., Inui, A., Fujisawa, T. & Ohtake, A.: "Indian childhood cirrhosis-like disease in a Japanese boy undergoing liver transplantation"J. Pediatr. Gastroenterol. Nutr. 29(5). 598-600 (1999)
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「研究成果報告書概要(欧文)」より
