1998 Fiscal Year Final Research Report Summary

Auti-inplawmatury effects of PUFA on wltured human wost cells

Research Project

Project/Area Number	09670838
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Pediatrics
Research Institution	Jikei University School of Medicine
Principal Investigator	NAGAKURA Toshikazu Jikei University School of Medicine, Assosiate Professor, 小児科, 助教授 (30125025)
Co-Investigator(Kenkyū-buntansha)	OBATA Toru Jikei University School of Medicine, Lecturer, DNA医学研究所・分子細胞生物学, 講師 (80119776)
Project Period (FY)	1997 – 1998
Keywords	cultured human / PGP_2 / EPA / COX-2 / cyfulcine
Research Abstract	We have investigated the role of EPA on on PGD2 generation in the cultured human mast cells with IgE-anti-IgE challenge incubation. Although EPA incubation did not affect histamine release from cultured human mast cells in response to challenge, it did decrease PGD2 generation by the inhibiting COX-2 pathway. In contrast, in the cell free homogenate of cultured human mast cells, EPA inhibited both COX-1 and COX-2 activities. Preincubation with EPA primarily affects the COX-2 pathway in cultured human mast cells and reduces PGD2 generation in response to IgE-anti-IgE challenge incubation. These findings suggest that COX-1 and COX-2 have different substrate flow systems in mast cells. Rhey also suggest that endogenous EPA diet supplemetation would reduce PGD2 production and could serve as an inflammatory substrate in human mast cells.