1998 Fiscal Year Final Research Report Summary
A study in the mechanism of cell fusion and giant cell fromation in skin diseases
| Project/Area Number |
09670875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Mie University |
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Principal Investigator |
MIZUTANI Hitoshi Mie Univ., Facul.Med., Associate Prof., 医学部, 助教授 (30115737)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Yasuhiko Mie Univ., Facul.Med., Prof., 医学部, 教授 (00022872)
TAKAHASHI Machiko Mie Univ.Hospital, Assistant, 医学部附属病院, 助手 (90273367)
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| Project Period (FY) |
1997 – 1998
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| Keywords | cell fusion / syncytium / CD98 / monocyte / giant cell / granuloma / membrane fusion |
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| Research Abstract |
To declare the mechanism of dermal multinuclear giant cells formation, we investigated fusion regulatory protein-1 (FRP-1) expression in the skin lesions of the systemic and cutaneous chronic inflammatory diseases. Immunohistological investigation using anti-FRP-1 monoclonal antibody revealed augmentation of membranous expression of FRP-1 in the inflammatory infiltrates of the early phaselesion of these skin diseases. According to the syncytium formation, the immunoreactive FRP-1 expression was down regulated significantly, and the membrane FRP-1 was no more detective in the matured giant cells. Subsequent, invitro cultured monocytes pulsed with a functional anti-FRP-1 monoclonal antibody induced immunoreactive FRP-1 expression, and its expression was augmented time dependently until the syncytium formation. In contrast, membrane FRP-1expression was downregulated significantly after cell fusion, and it was no more detective in the formed giant cells. These results confirmed involvement of the active FRP-1 membrane protein in the syncytium formation of monocytes and downregulation of membrane FRP-1 is a potent protective mechanism for over-sized giant cell formation.
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