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1998 Fiscal Year Final Research Report Summary

Making and analysis of a transjenic model of Mastocytosis and urticalia pigmentosa by the forced expression of steel factor in the skin.

Research Project

Project/Area Number 09670880
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionTOTTORI UNIVERSITY

Principal Investigator

KUNISADA Takahiro  Tottori University, Faculty of Medicine, Assistant Professor, 医学部, 助教授 (30205108)

Co-Investigator(Kenkyū-buntansha) MIZOGUCHI Masako  St.Marianna University, School of Medicine, Professor, 医学部, 教授 (30010250)
Project Period (FY) 1997 – 1998
Keywordssteel factor / transgenic mouse / mast cell / pigment cell / mastocytosis
Research Abstract

Transgenic mouse models for mastocytosis and urticalia pigmentosa were made by the forced expression of steel factor in the skin and the following results were obtained.
1. Mastocytosis was reproducibly expressed by the expression of soluble steel factor in the basal cells of skin. Membrane-bound type steel factor developed only melanosis, but not any signs of mastocytosis.
2. The mice that had shown mastocytosis were all developed melanocyte overgrowth in the skin, representing the symptom of urticalia pigmentosa, most common types of mastocytosis.
3. In vivo administration of blocking antibody of steel factor-c-Kit signaling completely abolished the maintenance of mast cells and melanocytes, indicating that increase of these cells were caused by the expression of the steel transgene.
4. Our mastocytosis models showed quicker and more extensive inflammatory response. This further implies that the model mice will be useful for the study of the roles of mast cells in inflammation.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Ogawa, M.: "Flt3/Flk-2 and c-kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse." Exp.Hematol.26. 478-488 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanio, Y.: "CD9 molecule expressed on stromal cells is involved in osteoclastgenesis." Exp.Hematol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki, H.: "Tooth-specific expression conferred by the regulatory sequences of rat dentin sialoprotein 2 gene in transgenic mice." Biochem.Biophys.Res.Commum.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辺見弘明: "SCFトランスジェニックマウス" 血液・腫瘍科. 36. 338-345 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto, A.: "Establishment and characterization of an immortal macrophage-like cell line inducible to differentiate to osteoclasts." Biochem.Biophys.Res.Commum.242. 703-709 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunisada, T.: "Hyperproliferaton of melanocytes ant mast cells caused by targeting expression of Steel factor to the skin." J.Exp.Med.187. 1565-1573 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa, K.: "Identification of areceptor tyrosine kinase involved in germ cell differentiation in planarians." Biochem.Biophys.Res.Commum.248. 204-209 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kunisada, K.: "Transgene expression of steel factor in the basal layer of epidermis promotes survival, proliferation, differentiation and migration of melanocyte precursors." Develpment. 125. 2915-2923 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, A.: "Establishment and characterization of pro-B cell lines from motheaten mutant mouse defective in SHP-1 protein tyrosine phosphatase" Immunol.Lett.63. 75-82 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki, H.: "Promotion of early osteoclastgenesis and B lymphoiesis in the bone marrow of transgenic rats with the env-pX gene of human T-cell lymphotropic virus type I." Oncogene. 17. 2955-2960 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa, M.: "Flt3/Flk-2 and c-kit are not essential for the proliferation of Blymphoid progenitor cells in the bone marrow of the adult mouse." Exp.Hematol.26. 478-488 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanio, Y.: "CD9 molecule expressed on stromal cells is involved in osteoclastgenesis." Exp.Hematol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki, H.: "Tooth-specific expression conferred by the regulatory sequences of rat dentin sialoprotein 2 gene in transgenic mice." Biochem.Biophys.Res.Commum.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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