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1998 Fiscal Year Final Research Report Summary

STUDY OF THERAPY OF GRAFT VERSUS HOST DISEASE AFTER BONE MARROW TRANSPLANTATION USING MICE MODEL

Research Project

Project/Area Number 09670892
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionYOKOHAMA CITY UNIVERSITY

Principal Investigator

AIHARA Michiko  YOKOHAMA CITY UNIV. SCH. OF MED., DERMATOLOGY, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (90231753)

Co-Investigator(Kenkyū-buntansha) AIHARA Yukoh  YOKOHAMA CITY UNIV. SCH. OF MED., PEDIATOLOGY, ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (50211686)
Project Period (FY) 1997 – 1998
KeywordsGVHD / ANIMAL MODEL / THERAPY
Research Abstract

We studied therapy of graft versus host disease after bone marrow transplantation using mice model. The effect of Dexamethasone palmitate (D-PAL) incorporated into lipid microspheres (D-PAL emulsion) on chronic GVHD was studied with animal model. BALB/c mice were lethally irradiated and administered spleen cells and bone marrow cells from B10.D2 mice . These mice are identical at the major histocompatibility complex (MHC, H-2 d) and at the minor lymphocyte stimulating locus (Mls, b). Weeks after transplantation, the BALB/c recipients exhibit hair loss, weight loss and atrophy of the lymph nodes and spleen and finally died due to chronic GVHD. D-PAL emulsion was administered to this GVHD mice model to investigate the effect of that drug on GVHD and to compare the effects of D-PAL emulsion with those of the water-soluble steroid, methylprednisolone (m-PSL). When mice were treated with D-PAL emulsion at day 2 after transplantation and afterward once every 3 or 4 weeks, 94% of them were survived at week 20 and only 12% of them showed hair loss. These data were significantly better than mice administered m-PSL( survival rate: 63%) or without any treatments (survival rate: 37%). Autopsy revealed that the survived recipient mice administered D-PAL emulsion showed no significant changes in skin, lymph nodes, spleen and liver, while no-treated recipients and recipients administered m-PSL showed tissue reduction and destruction with T cell infiltration in those tissues even though they survived.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 相原道子: "Effects of liposteroid on le***ns in **to*mmune MRL lpv/lpr mice"Journal of Dermatological Science. 16. 45-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石和万美子: "GVHD患者皮膚におけるアボトーンスの検討"日本皮膚科学会雑誌. 108. 1955-1963 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻 淳子: "GVHD患者皮膚部におけるアボトーンス関連遺伝子の検索"日本皮膚科学会雑誌. 109. 885-891 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michiko Aihara, Yukoh Aihara, Yuriko Takahashi, Hiroshi Nkajima: "Effects of liposteroid on skin lesions in autoimmune MRL lpr/lpr mice"J. Dermatological Science. 16. 45-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mamiko Ishiwa: "Detection of apoptosis in the epidermis of patients with graft-versus-host disease by TUNEL method"Jap J Dermatology. 108. 1955-1963 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junko Tuji: "Apoptosis associated mRNA expressions in graft-versus-host disease skin lesions"Jap J Dermatology. 109. 885-891 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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