1998 Fiscal Year Final Research Report Summary
Intervention of'non-insulin-dependent diabetes mellitus (NIDDM) by intravenous injection of 8-methoxypsoralen plus ultraviolet A (PUVA) -treated syngeneic splenocytes in KK-Ay mice
| Project/Area Number |
09670902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
OHNISHI Yoko Kansai Medical Universtiy, Department of Dermatology, Assintant, 医学部, 助手 (60278611)
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| Co-Investigator(Kenkyū-buntansha) |
HORIO Takeshi Kansai Medical University, Department of Dermatology, Professor, 医学部, 教授 (90026914)
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| Project Period (FY) |
1997 – 1998
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| Keywords | PHOTOCHEMOTHERAPY / ANIMAL MODEL / DIABETES MELLITUS / diabetes mellitus |
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| Research Abstract |
KK-Ay mice develop spontaneously positive glucosuria, impaired glucose tolerance, hyperinsulinemia, and hyperglycemia after 5-week (wk)-old of age. The effects of photochemotherapy on glucose intolerance were examined in KK-Ay mice. Once a week, a recipient KK-Ay mouse had intravenous injections of syngeneic splenocytes from obert-diabetic mice and treated with 8-rn ethoxypsoralen plus ultraviolet A light (PUVA). Intraperitoneal glucose tolerance tests (ipGTTs) revealed that the means of serum glucose and insulin levels in the PUVA-treated group were significantly lower than those of control groups, when recipients were older than 8-wk-old. Futherm ore, single injection with more than 1x107 cells from prediabetic 4-wk-old mice were also effective. Although the pathomechanism remains to be clarified, PUVA is an effective candidate to intervent the progression of NIDDM in KK-Ay m ice.
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