1998 Fiscal Year Final Research Report Summary
Modification of Multidrug Resistance by Magnetic Exposure Indicated from the Point of View of Nuclear Medicine
| Project/Area Number |
09670954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Keio University |
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Principal Investigator |
NAKAMURA Kayoko Keio University School of Medicine; Radiology; Assistant Professor, 医学部, 専任講師 (20124480)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Magnetic Exposure / Tumor / Chemosensitivity / Multidrug Resistance / Tc-99m-MIBI / P-glycoprotein |
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| Research Abstract |
The effects of magnetic field on the chemosensitivity of tumor cells were investigated by using Tc-99m-MIBI uptake; which indicates the function of P-glycoprotein. The cells lines studies were anaplastic thyroid cancer cell lines established from patients; human epidermoid cell KB-31; KB-31 transfected with mdr gene KB-G2; and MRP-cell line CA-500. By the strong magnetic exposure for a short period (one hour) the Tc-99m-MIBI uptake in some tumor cells (not all cells) was reduced. Under weak magnetic field the Tc-99m-MIBI uptake was increased in all tumor cells studied. These effects were not altered in the presence of anti-P-glycoprotein antibody or verapamil that inhibits the function of P-glycoprotein. It can be, therefore, concluded that the magnetic effects were different from cell to cell, and that these effects were not specific for P-glycoprotein. Magnetic exposure may be one of modifications that reverse multidrug resistance of tumor cells. Tc-99m-MIBI uptake in cells is a good indicator to assess the multidrug resistance modification.
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