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1998 Fiscal Year Final Research Report Summary

PCP(フェンシクリジン)投与により発現誘導される遺伝子cDNAのクローニング

Research Project

Project/Area Number 09670976
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionChiba University

Principal Investigator

KODAMA Kazuhiro  Psychiatry, Associate Professor, 医学部, 助教授 (90161942)

Co-Investigator(Kenkyū-buntansha) SHIRASAWA Hiroshi  Microbiology I,Professor, 医学部, 教授 (00216194)
Project Period (FY) 1997 – 1998
KeywordsPCP / Schizophrenia / glutamate dehydrogenase
Research Abstract

To clarify the molecular mechanism of phencyclidine (PCP) -induced schizophreniform psychosis in humans and behavioral abnormalities in ex-perimental animals, we used differential screening of a cDNA library from rat cerebral cortex treated with PCP.We identified a PCP-induced cDNA clone as the gene coding glutamate dehydrogenase (GDH), an enzyme central to glutamate metabolism. GDH mRNA levels significantly increased as imrnediate-early as 15 min following PCP administration in both cerebral cortex and cerebellum. This effect was observed even in the presence of protein synthesis inhibitor, cycloheximide (CHX). In contrast to a transient increase of c-fos expression, the elevation of GDH mRNA level lasted long up to 8 days after PCP injection. These results suggest that GDH mRNA induction may be involved in the pathology of PCP-induced psychosis, and that GDH can be one of the candidate genes which are vulnerable in subjects with schizophrenia.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Shimizu et al: "Expression, regulation and polymorphism of the mxi1 genes." Gene. 176. 45-48 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu et al: "Gultamate dehydrogenase mRNA is immediately lnduced after phencyclidine treatment in the rat brain." Schizophr. Res.25. 251-258 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu, E., Shirasawa, H., Kodama, K., Sato, T.and Simizu, B: "Expression, regulation and polymorphism of the mxil genes." Gene. 176. 45-48 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu, E., Shirasawa, H., Kodama, K., Kuroyanagi, H., Shirasawa, T., Sato, T.and Shimizu, B: "Glutamate dehydrogenase mRNA is immediately induced after phencyclidine treatment in the rat brain." Schizophr.Res.25. 251-258 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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