• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Expression of SDMF and atherosclerosis

Research Project

Project/Area Number 09671026
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionChiba University

Principal Investigator

MORISAKI Nobuhiro  Chiba Univ.Lecturer, 医学部, 講師 (40174411)

Co-Investigator(Kenkyū-buntansha) SAITO Yasushi  Chiba Univ.Professor, 医学部, 教授 (50101358)
SAITO Yasushi  Chiba Univ.Professor (50101358)
SAITO Yasushi  Chiba Univ.Professor (50101358)
SAITO Yasushi  Chiba Univ.Professor (50101358)
Project Period (FY) 1997 – 1998
Keywordssmooth muscle cells / SDMF / pheno type / migration / migration / Autocrine / otherosclerosis
Research Abstract

1. in vivo expression of SDMF : Rats were subjected to baloon catheter injury and SDMF mRNA was examined by the northern blot analysis. SDMF was not expressed in the arteries before injury, but expressed in them 14 days after injury. Moreover, in situ hybridization staining revealed that SDMF mRNA was expressed in the arteries not before injury but 14 days after injury. SDMF was selectively expressed in the thickened intima but not in the media of the arteries 14 days after injury. The expression pattern was similar to that of type I collagen.
2. regulation of SDMF gene : Cultured rat smooth muscle cells were used. Serum depletion from the culture medium stimulated SDMF mRNA expression by the northern blot analysis time-dependently from 12-48h. Next, serum depleted smooth muscle cells were stimulated by serum. On the first day, SDMF mRNA expression markedly decreased, then with increased cell density, SDMF mRNA expression increased, suggesting that cell density affects expression of SDMF gene. Finally, of various cytokines tested, only TGF-beta stimulated expression of SDMF.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tashiro J, Morisaki N et al: "Administvation of a small amrunt of lard enhances intineal thichening in the baloon catheter injury model without at fectry seram lipids" Scand J Clin Lab Invest. 58. 149-154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanzaki T, Morisaki N et al: "Role of latent TGF-β binding protein in vascular remodeling" Biocheur Biophys Res Commun. 246. 26-30 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morisaki N et al: "Angisgenic interaction between retina EC and PC from normal unddiabetic rabbits, and phenotype changes of diabetic cells" Cell Mol Biol. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro J,Morisaki N et al.: "administration of a small anmurt of lard enhances intinral thickening in the baloon catheter injury model without affecting serum lipids" Scand J Clin Lab Invest. 58. 149-154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanzaki T,Morisaki N et al.: "Role of latent TGF-beta binding protein in vascular remodeling" Biochem Biophys Res Commun. 246. 26-30 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morisaki N et al.: "angiogenie interaction between retinal EC and PC from normal and diabetic rabbits, and phenotype changes of diahetic cells" Cell Mol Biol. in press.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi