1998 Fiscal Year Final Research Report Summary
Investigation of stress response in osteoblasts
| Project/Area Number |
09671041
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Gifu University |
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Principal Investigator |
KOZAWA Osamu School of Medicine, Gifu University Associate Professor, 医学部, 助教授 (90225417)
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| Co-Investigator(Kenkyū-buntansha) |
UEMATSU Tohihiko School of Medicine, Gifu University Professor, 医学部, 教授 (50151832)
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| Project Period (FY) |
1997 – 1998
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| Keywords | heat shock protein / osteoblast / protein kinase C / mitogen-activated protein kinase / prostaglandin / endothelin / sphingosine 1-phosphate |
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| Research Abstract |
We investigated the effects of various physiological agonists on heat shock protein (HSP)27 induction in osteoblasts and the intracellular signaling system. We obtained the results as follows :
1. Prostaglandin F2alpha stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by prostaglandin F2alpha. In addition, p42/p44 mitogen-activated protein (MAP) kinase plays at a downstream from protein kinase C.
2. Endothelin-1 stimulates HSP27 induction in osteoblast-like MC3T3-El cells. The activation of protein kinase C is involeved in the HSP27 induction by endothelin-1. In addition, p38MAP kinase plays at a downstream from protein kinase C.
3. Sphingosine 1-phosphate, a metabolite of sphingomyelin, stimulates HSP27 induction in osteoblast-like MC3T3-E1 cells. p38MAP kinase is involved in the HSP27 induction by Sphingosine 1-phosphate.
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