1998 Fiscal Year Final Research Report Summary
Gene targeting of CD36, the major receptor for oxidized low density lipoproteins
| Project/Area Number |
09671055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Osaka University |
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Principal Investigator |
NOZAKI Shuichi Osaka University Medical School, Assistant Professor, 医学部, 助手 (30252646)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Makoto Osaka University Hospital, Medical Stuff, 医学部・附属病院, 医員
FUNAHASHI Tohru Osaka University Medical School, Assistant Professor, 医学部, 助手 (60243234)
TOMIYAMA Yoshiaki Osaka University Medical School, Assistant Professor, 医学部, 助手 (80252667)
YAMASHITA Shizuya Osaka University Medical School, Assistant Professor, 医学部, 助手 (60243242)
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| Project Period (FY) |
1997 – 1998
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| Keywords | CD36 / Atherosclerosis / Knockout mouse / Oxidized LDL / Receptors |
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| Research Abstract |
CD36 is one of the major receptor for oxidized low density lipoproteins (LDL).We have found that CD36 is expressed in macrophages resided in atherosclerotic lesions and its distribution in the lesions are different from that of scavenger receptor class A, in that CD36 is expressed dominantly in lipid-rich macrophages.To know the physiological function in vivo, we have tried to create the mice lacking this receptor in this research project.We have screened the C129 mouse genomic library with using murine CD36 cDNA as a probe and successfully cloned the genome encoding CD36.Based upon the restriction map of CD36 we made, we have constructed the replacement type targeting vector which lacks exon 1 containing the translation initiation codon of CD36.After electroporation of the construct DNA into embryonic stem (ES) cells, we have screened them with Neomycin and obtained five clones with homologous recombination, determined by Southern blot analysis.We have injected the positive ES clones into blastcysts and waiting for the chimeric mice's being born.
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