1998 Fiscal Year Final Research Report Summary
Transgenic approach for the study of physiological roles of pancreatic polypeptide
| Project/Area Number |
09671057
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Kobe University |
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Principal Investigator |
INUI Akio University Hospital Kobe University, Associate Professor, 医学部・附属病院, 講師 (80168418)
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| Project Period (FY) |
1997 – 1998
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| Keywords | pancreatic polypeptide / knockout mouse / feeding behabior / gastric motility / transgenic mouse / body weight regulatory |
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| Research Abstract |
Background & Aims : Pancreatic polypeptide (PP)is a 36-amino acid hormone produced by F cells within the pancreatic islets and the exocrine pancreas. The definitive function of PP in mammalian physiology still remains to be determined. This study was designed to examine the effects of chronic overexpression or deletion of PP through the development of PP transgenic mice. Methods : PP transgenic mice were created by using mouse PP cDNA under the control of the cytomegalovirus immediate early enhancer-chicken b-actin hybrid promoter (pCAGGS expression vector). Results : A unique line of transgenic mice were created that overexpress PP in the pancreatic islet with low levels of expression in other tissues including the brain. Plasma PP concentrations were more than 20 times higher than those of control littermates. PP transgenic mice gained less weight with specifically reduced food intake and fat mass compared to controls, results which were more evident in male than in female mice. The transgenic mice exhibited a reduced rate of gastric emptying of a solid meal but had normal oxygen consumption and fasting leptin levels. Immunoneutralization with anti-PP antiserum reversed the phenotypic changes of transgenic animals. Conclusions : These results indicate that PP could be involved in feeding and body weight regulation partly through regulation of gastric emptying.
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