1998 Fiscal Year Final Research Report Summary
Regulatory mechanism to tissue factor gene expression by its methylation/demethylation
| Project/Area Number |
09671108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAKAMURA Shin Primate Research Institute, KYOTO UNIVERSITY,Instructor, 霊長類研究所, 助手 (10101255)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hideo Mie University, School of Medicine, Instructor, 医学部, 助手 (40158704)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Tissue Factor / Methylation / Demethylation / Gene expression / Regulatory mechanism / Seminal vesicle / Testosterone / TF-mRNA / 精嚢 |
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| Research Abstract |
Tissue Factor (TF) is a factor VII/VIIa receptor that initiates blood coagulation. TF is suggested to have other functions such as involvement in embryogenesis, angiogenesis, tumor metastasis, and/or signal transdution. TF gene expression is regulated by several is-elements of AP-1, kappaB, SP-1 and Erg-1 and transcriptional factors to bind these cis-elements. Another regulatory machinery to TF gene expression is expected because there are a lot of CG island specific site for methylation at cytosine residues.
In this studies we examined a possible regulation mechanism of TF gene expression through methylation and/or demethylation of its promoter region. We also investigated a relationship between differentiation and de-differentiation/transformation of several cells and methylation/ demethylation of TF gene.
We established a competitive RT-PCR which is enable to quantitative measurement of TF m-RNA expressed in several normal or transformed cells using specific primer sets. We also established sensitive sandwich enzyme-linked immuno-sorbent assay to measure TF antigen produced in these cells.
In normal blood cells monocytes and granulocytes expressed TF but lymphocytes did not. Marked different expression of TF gene in monkey male reproductive tissues. Seminal vesicle specifically expressed very high level of TF-mRNA.These suggest TF gene expression should regulated by methylation/demethylation during differentiation of leukocytes from common precursor and tissue speculation.
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[Publications] R.Watanabe, M.Murata, N.Takayama, M.Tokuhira, M.Kizaki, S.Okamoto, Y.Kawai, K.Watanabe, H.Murakami, M.Kikuchi, S.Nakamura, Y.Ikeda: "Long-Term Follow-up of Hemostatic Molecular Markers during Remission Induction Thetapy with All-trans Retinoic Acid for Acute Promyelocytic Leukemia." Thromb.Haemost.77. 641-645 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] K.Kaikita, H.Ogawa, H.Yasue, M.Takeya, K.Takahashi, T.Saito, K.Hayasaka, K.Horiuchi, A.Takizawa, Y.Kamikubo, S.Nakmamura: "Tissue Factor Expression on Macrophages in Coronary Plaques in Patients with Unstable Angina" Arterioscler.Thromb.Vasc.Biol.17. 2232-2237 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] H.Suefuji, H.Ogawa, H.Yasue, K.Kaikita, H.Soejima, T.Motoyama, Y.Mizuno, S.Oshima, T.Saito, I.Tsuji, K.Kumeda, Y.Kamikubo, S.Nakamura.: "Increased Plasma Tissue Factor Levels in Acute Myocardial Infarction." Am.Heart J.134. 253-259 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] J.M.Morrissey, H.Agis, S.Albrecht, F.Dignat-George, T.S.Edgington, T.Luther, M.Muller, M.Mutin, S.Nakamura, P.Valent, G.M.Vercellotti: "CD142 (tissue factor)" Workshop panel report, Leukocyte Typing IV,White Cell Differentiation Antigens. eds., T.Kishimoto, et al.742-746 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] K.Misumi, H.Ogawa, H.Yasue, H.Soejima, H.Suefuji, K.Nishiyama, K.Takagoe, K.Kugiyama, I.Tsuji, K.Kumeda, S.Nakamura: "Comparison of Plasma Tissue Factor Levels in Unstable and Stable Angina Pectoris" Am.J.Cardiol.81. 22-26 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] H.Wada, Y.Mori, M.Shimura, k.Hiyoyama, M.Loka, T.Nakasaki, M.Nishikawa, M.Nakano, K.Kumeda, T.Kaneko, S.Nakamura, H.Shiku.: "Poor Outcome in Disseminated Intravascular Coagulation or Thrombotic Thrombocytopenic Purpura Patients with Sever Vascular Ebdothelial Cell Injuries." Amer.J.Hematol.58. 189-194 (1998)
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「研究成果報告書概要(欧文)」より
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