1998 Fiscal Year Final Research Report Summary
Wilms tumor gene(WT1)expression in leukemic and normal hematopoietic stem cells.
| Project/Area Number |
09671110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
OGAWA Hiroyasu Osaka University Medical School, Associate Professor, 医学部, 助教授 (80194447)
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| Co-Investigator(Kenkyū-buntansha) |
TATEKAWA Toyoshi Osaka University Hospital, Medical Staff, 医学部・附属病院, 医員
OJI Yusuke Osaka University Medical School, Assistant Professor, 医学部, 助手 (20294100)
SOMA Toshihiro Osaka University Medical School, Assistant Professor, 医学部, 助手 (40273619)
OKA Yoshihiro Osaka University Medical School, Assistant Professor, 医学部, 助手 (20273691)
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| Project Period (FY) |
1997 – 1998
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| Keywords | WT1 gene / minimal residual disease / hematopoietic stem cell / Leukemia |
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| Research Abstract |
As Wilms' tumor gene (WT1) is highly expressed in bone marrow and blood specimens in all patients with leukemia, minimal residual leukemia (MRD) can be estimated by the quanlitation of the gene expression with RT-PCR method.Low level expression of WT1 gene in CD34 fraction of normal bone marrow cells may result in reducing the sensitivity of the detection of MRD by WT1 assay.In allogeneic bone marrow transplantation setting, CD34 fraction and WT1 levels of the bone marrow cells were monitored in patients with lymphoma who did not expressed WT1 gene.As a result, the percentage of the normal CD34 fraction and WT1 gene expression level derived from the fraction concurrently decreased to less than one tenth.On the other hand, WT1 gene expression in leukemic cells is increased in relapse.Therefore, this MRD detection system is very useful for the diagnosis of early molecular relapse in patients with leukemia who were treated with bone marrow transplantation.
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[Publications] Inoue K,Tamaki H,Ogawa H,Oka Y,Soma T,Tatekawa T,Oji Y,Tsuboi A,Kim EH,Kawakami M,Akiyama T,Kishimoto T,and Suigyama H.: "Wilms'tumor gene(WT1)competes with differentiation-inducing signal in hematopoietic progenitor cells." Blood. 91. 2969-2976 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tamaki H,Ogawa H,Ohyashiki K,Oyhashiki JH, Iwama H,Inoue K,Soma T,Oka Y,Tatekawa T,Oji Y,Tsuboi A,Kim EH,Kawakami M,Fuchigami K,Tomonaga M,Toyama K,Aozasa K,Kishimoto T,and Sugiyama H.: "The Wilms' tumor gene WT1 is a good marker for diagnosis of disease progression of myelodysplastic syndromes." Leukemia. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tsuboi A,Oka Y,Ogawa H,Elisseeva AA,Tamaki H,Oji Y,Kim EH,Soma T,Tatekawa T,Kawakami M,Kishimoto T,and Sugiyama H.: "Constitutive expression of the Wilms'tumor gene WT1 inhibits the differentiation of myeloid progenitor cells but promotes their proliferation in response to granulocyte-colony stimulating factor (G-CSF)." Leukemia Research. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Oji Y,Ogawa H,Tamaki H,Oka Y,Tsuboi A,Kim EH,Soma T,Tatekawa T,Kawakami M,Asada M,Kishimoto T,and Sugiyama H.: "Expression of the Wilms'tumor gene WT1 in solid tumors and its involvement in tumor cell growth." Japanese Journal of Cancer Research.
Description
「研究成果報告書概要(欧文)」より
