1998 Fiscal Year Final Research Report Summary
Mutation analysis of c-kit and c-mpl genes in chronic myelogenous leukemia
| Project/Area Number |
09671137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
INOKUCHI Koiti Nippon Medical School, Dept.of Internal Medicine, Lecturer, 医学部, 講師 (10203267)
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| Project Period (FY) |
1997 – 1998
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| Keywords | LEUKEMOGENESIS / c-kit / c-mpl / mutation / CML |
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| Research Abstract |
Leukemogenesis was investigated with molecular biological methods. Especially, abnormalities of c-kit and c-mpl genes in chronic myelogenous leukemia (CML) were detected. Some CML patients had abnormal isoform of c-mpl transcript, and others had a point mutation of c-kit genes. Minor type bcr-abl transcript was revealed to make CML patients atypical clinicalcourses other than typical CML.Acute lymphoblastic leukemia with atypical P180 BCR/ABL protein was also atypical clinical course with chemo-resistance. Usually, there are three isoforms of c-mpl transcript In normal control, patients with idiopathic thrombocytopenic purpura and myelodysplastic syndromes (MDS). But, CML patients had abnormal c-mpl transcript without half of exon 11. This abnormality of c-mpl may be clinical heterogeneity of thrombocytosis. Some CML patients had point mutation of codon 541. These CML had remarkable leukocytosis. The same point miutation of codon 541 was occurred in some CML patient at blastic crisis, whose skin has no mutation of codon 541. Biological function of abnormalities of these c-kit and c-mpl proteins are investigating with mutagenesis method.
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