2000 Fiscal Year Final Research Report Summary
Analysis of lymphocyte maturation after purified hematopoietic stem cell transplantation and induction of tumor immunity.
| Project/Area Number |
09671145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | AICHI CANCER CENTER |
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Principal Investigator |
MORISHIMA Yasuo RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (20220056)
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| Co-Investigator(Kenkyū-buntansha) |
KOGAMI Yoshitoyo RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (30270721)
OGURA Michinori RESEARCH INSTITUTE, AICHI CANCER CENTER, RESEARCHER, 研究所, 研究員 (40231229)
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| Project Period (FY) |
1997 – 1998
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| Keywords | hematopoietic stem cell transplantation / CD34 positive stem cell. / tumor immunity / maturation of lymphocyte. / EB virus. / NK / T cell lymphoma / cell line |
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| Research Abstract |
Immunologocal recovery after purified hematopoietic stem cell transplantation was analyzed in 15 patients with hematological malignancy. 2.3x 10＼o＼ac (＼s＼up 90,7)/kg (median) of CD34 positive hematopoietic stem cells were purified by Isolex System using anti-CD34 monoclonal antibody and immuno-magnetic beads. Lymphocyte/NK cell subpopulations in peripheral blood were monitored after transplantation. Increase of CD57+, DC8+ cell was observed after one month of transplantation. One patient transplanted by preconditioning of total body irradiation (TBI) and melphalan showed a remarkable increase of CD57+, CD8+ cells after cytomegarovirus infection, and its increse was persited for more than 8 months with abnormarity of chromosome in 2/20 PB cells and oligoclonal TCR rearrangement bands. Including this patient, high frequency of opportunistic infections was observed in patients treated with TBI and CD34 positive selection. These findings suggest that add-back of T cells is necessary in order to reconstitute the immunological deficient state after CD34 positively selected transplantation
In order to make the model of adoptive immunotherapy for malignangt lymphoma, we established a IL-2 dependent culture cell line from nasal type NK/T cell lymphoma. This cell line was CD2+, sCD3-, CD3ε+, CD8-, CD16+, CD56+, CD94+, CD158a, b-and TIA1+, granzyme B+. Clonal integration of EB virus was detected, and type II latency pattern (LMP-1+, EBNA2-) of EB virus infection was observed. This cell line will be a useful target cell for the induction of cytotoxic T cells against EB virus cell suface antigen.
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