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1998 Fiscal Year Final Research Report Summary

Isolation and characterization of pathogenic antigen in experimental membranous nephropathy.

Research Project

Project/Area Number 09671148
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionGunma University

Principal Investigator

NARUSE Takuji  Gunma University, Faculty of Medicine, Professor, 医学部, 教授 (50010369)

Project Period (FY) 1997 – 1998
KeywordsMembranous Nephropathy / Experimental Nephritis / Heymann Nephritis / Renal Tubular Antigen / Immune Complex / Autoimmune Disease
Research Abstract

Heymann nephritis is an experimental model of human idiopathic membranous nephropathy. As Heymann nephritis can be induced by immunizing rats with proteins derived from renal tubule, there have been many attempts to isolate and characterize a nephrito- genic antigen from crude mixes of brush border antigens.
Kerjaschki et al. demonstrated that a nephritogenic 330-kD glycoprotein (gp330). presented in the brush border of renal proximal tubule as well as on the glomerular epithelial surface, and proposed that Heymann nephritis is caused by locally formed. antigen-antibody complexes on the epithelial surface of glome- rulus (in situ immune complex formation theory), rather than the deposition of circulating immune complex.
We have isolated a nephritogenic 120-kD (gp120) antigen from rat tubule brush border. The gpl20 induces rat Heymann nephritis. A monoclonal antibody to the antigen reacted exclusively with the brush border, not with glomerular epithelia on direct immunofluorescence and immunoelectron microscopy.
cDNA cloning and sequencing of the nephritogenic. Gp120 antigen showed similarity to that of low density lipoprotein receptor (LDLR) which was also reported as a homologue of gp330 antigen.
Nephritogenic major epitope in gp330 has been reported to localize in aminoacids 1160-1205 on LDLR, whereas, our gp120 antigen localizes in aminoacids approximately between 1534- 2910.
As we have obtained enough cDNA data for our gp120 antigen, we are preparing several bacterial recombinant proteins. Using these recombinant proteins, we can confirm which part is antigenic, and will enable us to select the most important region in gp120 antigen.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Keiju Hiromura: "Tubulointerstitial mast cell infiltration in glomerulo-nephritis." American Journal of Kidney Diseases. 32. 593-599 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miwako Mitsuhashi: "Levels of serum glycosaminoglycans in renal failure." Res Commun Mol Pathol Pharmacol. 99. 225-232 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideo Kanai: "Role of serotonin in nephrotoxic serum nephritis in WKY rats." Journal of Laboratory and Clinical Medicine. 129. 557-566 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yosito Tsukada: "Relapsing membranous nephropathy with a good response to steroid." Nephron. 76. 218-221 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Maezawa: "The kinetics of the pathogenic pronase-digested renal proximal tubular antigen and antibody in rat active Heymann nephritis." Nephron. 71. 448-453 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yosito Tsukada: "A major pathogenic antigen of Heymann nephritis is present exclusively in the renal tubule brush border." Clinical and Experimental Immunology. 96. 303-310 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiji Hiromura: "Tubulointerstitial mast cell infiltration in glomerulonephritis." Am J Kidney Dis. 32. 593-599 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideo Kanai: "Role of serotonin in nephrotoxic serum nephritis in WKY rats." J Lab Clin Med. 129. 557-566 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yosito Tsukada: "Relapsing membranous nephropathy with a good response to steroid." Nephron. 76. 218-221 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Maezawa: "The kinetics of the pathogenic pronase-digested renal proximal tubular antigen and antibody in rat active Heymann nephritis." Nephron. 71. 448-453 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yosito Tsukada: "A major pathogenic antigen of Heymann nephritis is present exclusively in the renal tubule brush border." Clin Exper Immunol. 96. 303-310 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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