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1998 Fiscal Year Final Research Report Summary

Cadherin superfamily in intercellular junctions of podocytes in the kidney

Research Project

Project/Area Number 09671156
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNiigata University

Principal Investigator

YAOITA Eishin  Niigata University, School of Medicine Assistant, 医学部, 助手 (00157950)

Co-Investigator(Kenkyū-buntansha) KIHARA Itaru  Niigata University, School of Medicine Prof., 医学部, 教授 (80018324)
YAMAMOTO Tadashi  Niigata University, School of Medicine Associate Proc., 医学部, 助教授 (30092737)
Project Period (FY) 1997 – 1998
Keywordskidney / glomerulus / epithelial cell / podocyte / cadherin / protocadherin / FAT
Research Abstract

During a search for cadherin-related molecules expressed in rat glomeruli by PT-PCR with degenerate primers, we have isolated cDNA encoding a rat homologue of human FAT.
FAT, a new member of the cadherin superfamily, resembles the Drosophila tumor suppressor fat. It is a large transmembrane protein with 34 tandem cadherin-like repeats, five EGF-like repeats, and a laminin A-G domain, indicating its potential role in cell-cell adhesion and cell proliferation. To localize FAT and to speculate its role in the kidney, we examined its expression in the rat neonatal and adult kidney in the physiological and pathological conditons by using ribonuclease protection assay (RPA), in situ hybridization(ISH) and immunostaining.
Comparison of the deduced amino acid sequences showed more than 85% identical residues in rat and human FAT, especially, 100% identical in C-terminus of FAT of the both species. Affinity-purified antibody against FAT C-terminus was prepared. RPA revealed significant signals in RNA from glomeruli of the normal rat kidney. By ISH, FAT expression was observed in podocytes. Immunostaining for FAT was coincident with that of 5.1.6, which is antibody specific for slit diaphragm. In PAN nephrosis, conspicuous increase in FAT expression in glomeruli was shown by RPA, ISH, and immunostaining. In the neonatal kidney, FAT was observed in podocytes throughout the development from S-shaped body to mature glomeruli.
The above findings suggest that FAT is one of basic constitutents of intercellular junctions of podocytes.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 矢尾板永信: "Transient expression of desmoplakins in murine glomerulogenesis" Cell-Cell Interaction in Kidney, NIHON-IGAKUKAN. 29-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木原達 他: "Podocyte detachment and epithelial cell reaction in focal segmental glomerulosclerosis with cellular variants" Kidney Int.52. S・171-S・176 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢尾板永信: "糸球体上皮細胞培養" Annual Review 腎臓1998. 25-30 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤眞 他: "Involvement of R-cadherin in the early stage of glomerulogenesis" J.Am.Soc.Nephrol.9. 1234-1241 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢尾板永信: "糸球体におけるカドヘリン" 発達腎研究会誌. 6(1). 12-17 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢尾板永信 他: "Identification of renal podocytes in multiple species" Histochem Cell Biol. 111. 107-115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yaoita E,Franke WW,Yamamoto T,Kawasaki k, Kihara I: "Identification of renal podocytes in multiple species : higher vertebrates are vimentin positive/lower vertyebrates are desmin positive" Histochem Cell Biol. 111. 107-115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto S,Yaoita E,Matsunami H,Kondo D,Yamamoto T,Kawasaki K,Arakawa M,Kihara I: "Involvement of R-cadherin in the early stage of glomerulogenesis" J Am Soc Nephrol. 9. 1234-1241 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose S,Yamamoto T,Feng T,Yaoita E,Kawasaki K,Goto S,Fujinaka H,Wilson * CB,Arakawa M,Kihara I: "Expression and localization of cyclooxygenase isoforms and cytosolic phospholipase A2 in anti-Thy-1 glomerulonephritis" J Am Soc Nephrol. 9. 408-416 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kihara I,Tsuchida S,Yaoita E,Yamamoto T,Hara M,Yanagihara T,Takada T: "Podocyte detachment and epithelial cell reaction in focal segmental glomerulosclerosis with cellular variants." Kidney International. 52. S-171-S-176 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujinaka H,Yamamoto T,Feng L,Kawasaki K,Yaoita E,Hirose S,Goto S,Wilson CB,Uchiyama M,Kihara I.: "A crucial role of CD8-positive lymphocytes in glomerular expression of ICAM-1 and cytokines in crescentic glomerulonephritis of WKY rats" J Immunol. 158. 4978-4983 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yaoita E,Goto S: "Transient expression of desmoplakins in murine glomerulogenesis" Cell-Cell Interaction in Kidney. 29-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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