1998 Fiscal Year Annual Research Report

チネイシンによる糸球体障害修復機構の研究

Research Project

Project/Area Number	09671187
Research Institution	The Institute of Physical and Chemical Research (RIKEN)
Principal Investigator	日下部守昭理化学研究所, 実験動物室, 室長(研究職) (60153277)
Co-Investigator(Kenkyū-buntansha)	吉木淳理化学研究所, 実験動物室, 研究員 (40212310) 池郁生理化学研究所, 実験動物室, 先任研究員 (40183157)
Keywords	糸球体腎炎 / テネイシン / 創傷治癒 / サイトカン / TGF-B1 / PDGF-BB / 細胞外マトリックス / 遺伝子欠損マウス
Research Abstract	1) 本年度は、前年度の成果の論文発表を行った(別刷り代支払)。2) ハブ毒で誘発した実験的糸球体腎炎モデルおよびテネイシン欠損マウスを用いて、テネイシンがメサンギウム細胞の増殖に必要なシグナル伝達を調整していることを示したが、正常マウスにハブ毒によって糸球体腎炎を誘発した後、テネイシン機能を中和する抗-テネイシン抗体を投与すると、対象群では時間経過とともに腎臓の機能が正常に戻るが、投与群ではテネイシン欠損マウスの場合と同様、腎臓の機能低下(アルブミン尿、血中尿素量の増加)が観察された。以上のことより、糸球体腎炎の修復過程においてテネイシンは、メサンギウム細胞の増殖に関連した重要な働きを持っていることが再確認された。 3) テネイシン欠損マウスから樹立したメサンギウム細胞の培養細胞株を用いて、テネイシンのPDGFやTGF-βの受容体発現に対する影響をPCR法を用いて解析したところ、テネイシンは、これらの受容体の発現調節に関与しているらしいことを示唆する結果を得た。現在は、この現象を更に詳細に検討中である。

Research Products
(13 results)

All Other

All Publications (13 results)

[Publications] Kubota, Y., et al.: "Spaclal and temporal changes in chondroitin sulfate distribution in the sclerotome play an essential role in the formation of migration patterns of mouse neural crest cells." Devel.Dyn.214. 55-65 (1999)
[Publications] Hashimoto, H.et al.: "Simultaneous observation of capillary nets and tenascin in intestinal villi." Anat Rec.250. 488-492 (1998)
[Publications] Nakao, N.et al.: "Tenascin C promotes healing of Habu-Snake Venom-induced glomerulonephritis.Studies in knockout congenic mice and in culture" Am.J.Pathol.152(5). 1237-1245 (1998)
[Publications] Shibata, H.et al.: "A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene:An association with a short tandem repeat and a hypermethylated region" Genomics. 49. 30-37 (1998)
[Publications] Hashimoto, H.et al.: "Three-dimensional analysis of the developing pituitary gland in the mouse" Devel.Dynam.212. 157-166 (1998)
[Publications] Hashimoto, H.et al.: "Three-dimensional investigation of vascular nets by fluorochrome-labelled angiography" Microvascular Res.55. 179-183 (1998)
[Publications] Sasaki, N.et al.: "Characterization of gene expression in mouse blastocyst using single pass sequencing of 3995 clones" GENOMICS. 49. 167-179 (1998)
[Publications] Yoshiki, A.and Kusakabe, M.: "Cerebellar histogenesis as seen in identified cells of normal-reeler mouse chimeras" Int.J.Dev.Biol.42. 695-700 (1998)
[Publications] Koyama Y-I.et al.: "Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse" J.Inv.Derm.111(6). 930-935 (1998)
[Publications] Dvorak, P.et al.: "Embryoglycans regulate bloiogical activity of FGF-2 to embryonic stem cells." J.Cell.Sci.111. 2945-2952 (1998)
[Publications] Steindler, D.A.et al.: "Boundary molecules during brain development, injury, and persistent neuro genesis-in vivo and in vitro studies." Progress in Brain Research. 117. 177-196 (1998)
[Publications] 日下部守昭: "細胞接着のしくみと疾患、イラスト医学&サイエンスシリーズ、" 羊土社, 127 (1998)
[Publications] Kusakabe, M., & Sakakura, T.: "“Extracellular Matrix-Cell Interaction:Molecules to Diseases,"" Japan Sci.Soc.Press, Tokyo/S.Karger, Basel,382 (1998)

1998 Fiscal Year Annual Research Report

チネイシンによる糸球体障害修復機構の研究

Principal Investigator

日下部 守昭 理化学研究所, 実験動物室, 室長(研究職) (60153277)

Research Products

[Publications] Kubota, Y., et al.: "Spaclal and temporal changes in chondroitin sulfate distribution in the sclerotome play an essential role in the formation of migration patterns of mouse neural crest cells." Devel.Dyn.214. 55-65 (1999)

[Publications] Hashimoto, H.et al.: "Simultaneous observation of capillary nets and tenascin in intestinal villi." Anat Rec.250. 488-492 (1998)

[Publications] Nakao, N.et al.: "Tenascin C promotes healing of Habu-Snake Venom-induced glomerulonephritis.Studies in knockout congenic mice and in culture" Am.J.Pathol.152(5). 1237-1245 (1998)

[Publications] Shibata, H.et al.: "A methylation imprint mark in the mouse imprinted gene Grf1/Cdc25Mm locus shares a common feature with the U2afbp-rs gene:An association with a short tandem repeat and a hypermethylated region" Genomics. 49. 30-37 (1998)

[Publications] Hashimoto, H.et al.: "Three-dimensional analysis of the developing pituitary gland in the mouse" Devel.Dynam.212. 157-166 (1998)

[Publications] Hashimoto, H.et al.: "Three-dimensional investigation of vascular nets by fluorochrome-labelled angiography" Microvascular Res.55. 179-183 (1998)

[Publications] Sasaki, N.et al.: "Characterization of gene expression in mouse blastocyst using single pass sequencing of 3995 clones" GENOMICS. 49. 167-179 (1998)

[Publications] Yoshiki, A.and Kusakabe, M.: "Cerebellar histogenesis as seen in identified cells of normal-reeler mouse chimeras" Int.J.Dev.Biol.42. 695-700 (1998)

[Publications] Koyama Y-I.et al.: "Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse" J.Inv.Derm.111(6). 930-935 (1998)

[Publications] Dvorak, P.et al.: "Embryoglycans regulate bloiogical activity of FGF-2 to embryonic stem cells." J.Cell.Sci.111. 2945-2952 (1998)

[Publications] Steindler, D.A.et al.: "Boundary molecules during brain development, injury, and persistent neuro genesis-in vivo and in vitro studies." Progress in Brain Research. 117. 177-196 (1998)

[Publications] 日下部守昭: "細胞接着のしくみと疾患、イラスト医学&サイエンスシリーズ、" 羊土社, 127 (1998)

[Publications] Kusakabe, M., & Sakakura, T.: "“Extracellular Matrix-Cell Interaction:Molecules to Diseases,"" Japan Sci.Soc.Press, Tokyo/S.Karger, Basel,382 (1998)

日下部守昭理化学研究所, 実験動物室, 室長(研究職) (60153277)