• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Study on a possible function of tenascin-C in wound healing of glomerulonephritis

Research Project

Project/Area Number 09671187
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionThe Institute of Physical and Chemical Research (RIKEN)

Principal Investigator

KUSAKABE Moriaki  RIKEN,Division of Experimental Animal Research, Chief Scientist, 実験動物室, 室長(研究職) (60153277)

Co-Investigator(Kenkyū-buntansha) YOSHIKI Atsushi  RIKEN,Division of Experimental Animal Research, Research Scientist, 実験動物室, 研究員 (40212310)
IKE Fumio  RIKEN,Division of Experimental Animal Research, Senior Research Scientist, 実験動物室, 先任研究員 (40183157)
Project Period (FY) 1997 – 1998
Keywordstenascin / extracellular matrix / wound healing / knockout mice / reversible kidney injury / cytokines / 細胞外マトリックス / 遺伝子欠損マウス
Research Abstract

Mice without the gene for tenascin-C, a multifunctional extracellular matrix protein expressed in many important biological events, including wound healing, did not show any phenotype. However, it is now obvious that the phenotype of deletion of one gene frequently depends on the genetic background. Therefore, we have newly generated tenascin-C knockout mice(KO)by backcrossing original KO into three congenic lines : C57BL/6N, BALB/cA, and GRS/A (GR). And we investigated the disease course of reversible kidney injury, Habu-snake venom-induced proliferative glomerulonephritis. In all strains, the disease was more severe in KO, but the severity varied with the strain. The KO-GR showed abnormal regenerative reactions, including reduced proliferation of mesangial cells, key players in glomerulonephritis, and reduced production of some kinds of cytokines and matrices, leading to poor formation of granulation tissue. In culture, the mesangial cells from the KO-GR had the same potential for proliferation and response to cytokines as controls, but interestingly, to achieve this potential, they required contact with tenascin-C.These reactions were blocked by an anti-tenascin monoclonal antibody. The results of the present study, the first report showing the most dramatic phenotype so far discovered, have strongly suggested the importance of tenascin-C in the resolution of the renal inflammation and that of the genetic background on which the KO was developed.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Kubota,Y.,et al.: "Spacial and temporal changes in chondroitin sulfate distribution in the sclerotome play an essential role in the formation of migration patterns of mouse neural crest cells." Devel.Dyn.214. 55-65 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,H.,et al.: "Simultaneous observation of capillary nets and tenascin in intestinal villi." Anat Rec.250. 488-492 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakao,N.et al.: "Tenascin-C promotes healing of Habu-Snake Venom-induced glomerulonephritis. Studies in knockout congenic mice and in culture" Am.J.Pathol.152(5). 1237-1245 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibata,H.et al.: "A methylation imprint mark in the mouse imprinted gene Grfl/Cdc25Mm locus shares a common feature with the U2afbp-rs gene:An association with a short tandem repeat and a hypermethylated region" Genomics. 49. 30-37 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimto,H.et al.: "Three-dimensional analysis of the developing pituitary gland in the mouse" Devel.Dynam.212. 157-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,H.et al.: "Three-dimensional investigation of vascular nets by fluorochrome-labelled angiography" Microvascular Res.55. 179-183 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sasaki,N.et al.: "Characterization of gene expression in mouse blastocyst using single-pass sequencing of 3995 clones" GENOMICS. 49. 167-179 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yohsiki,A.and Kusakabe,M.: "Cerebellar histogenesis as seen in identified cells of normal-reeler mouse chimeras" Int.J.Dev.Biol.42. 695-700 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koyama Y-I.et al.: "Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse" J.Inv.Derm.111(6). 930-935 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dvorak,P.et al.: "Embryoglycans regulate biological activity of FGF-2 to embryonic stem cells." J.Cell.Sci.111. 2945-2952 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Steindler,D.A.et al.: "Boundary molecules during brain development,injury,and persistent neuro genesis-in vivo and in vitro studies." Progress in Brain Research. 117. 177-196 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 日下部守昭: "細胞接着のしくみと疾患、イラスト医学&サイエンスシリーズ、" 羊土社, 127 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusakabe,M.,&Sakakura,T.: "“Extracellular Matrix-cell Interaction:Molecules to Diseases,"" Japan Sci.Soc.Press,Tokyo/S.Karger,Basel,382 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.JYO,et.al.: "Expression of tenascin in mesangial injury in experimental glomerulonephritis." Exp.Nephrol.5. 423-428 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.NAKAO,et.al.: "Tenascin-C promotes healing of Habu-Snake Venom-induced glomerulonephritis. Studies in knockout congenic mice and in culture" Am.J.Pathol.152(5). 1237-1245 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.NAKAO,et.al.: "Reversible type of Habu-snake induced murine glomerulonephritis was irreversible in tenascin knockout mice" XXXV Congress of the European Renal Association European Dialysis and Transplant Association 1998 June 6-9.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.KUSAKABE,et.al.: "Extracellular matrix in tissue remodeling : Tenascin-C as a modulator in cell-matrix interactions." "Extracellular Matrix-Cell Interaction : Molecules to Diseases, " Y.Ninomiya, Reino, B., and Ooyama, T., eds., pp87-107., Japan Sci.Soc.Press, Tokyo/S.Karger, Basel. (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.KUSAKABE: "Analysis of gene function using experimental animal and animal experiment" Kansai Journal of Laboratory Animals Kansai Laboratory Animal Research Association 1998 December No.19.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi