1998 Fiscal Year Final Research Report Summary
The mutation enhancement in human cell lines by serum factors from pancreatic cancer patients
| Project/Area Number |
09671209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
YAMAMORI Hideo Chiba Univ.Med.Surgery Lecturer, 医学部, 講師 (00166836)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Tsuguhiko Chiba Univ.Med.Surgery, Associate Professor, 医学部, 助教授 (70143310)
SUZUKI Nobuo Chiba Univ.Med.Biochemi., Professor, 医学部, 教授 (90111426)
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| Project Period (FY) |
1997 – 1998
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| Keywords | pancreatic cancer / serum factor / K-ras / mutation / protease |
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| Research Abstract |
In pancreatic cancer cells base institution mutation is very highly detected. We have reported that sera from pancreatic cancer patients have the ability to enhance the mutation frequency. In the present research effects of the sera on protein and DNA metabolism were studied, in order to clarify mechanism of the enhancement activity. We have already found that human interferon induce the activity of antipain-sensitive proteases in early steps of suppression of the mutation incidence. Thus, fibrinolysis activity was estimated using ^<125>I-fibrin as substrates in cells pretreated with serum factors from pancreatic cancer patients and then irradiated with UV.The factors were obtained by a color-column chromatography method. The fraction sample was obtained which enhance frequencies of ouabain-resistant phenotypic mutation induced by UV.The fraction sample also showed the ability to enhance frequencies of K-ras codon 12 mutation detected by the PCR-based differential dot-blot hybridization analysis. Furthermore the sample enhance the proteases activity induced by UV On the other hand, induction of several genes expression was identified by the RT mRNA differential display method.
Therefore, it was suggested that serum factors from pancreatic cancer patients enhance K-ras codon 12 mutation possibly via proteases induction followed by several genes expression.
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[Publications] Isogai, E, Ishijima, S., Sonoda, T., kita, K., Suzuki, H., Haseagwa, R., Yamamori, H., Takakubo, Y., and Suzuki, N.: "Protease activation following UV irradiation is linked to hypomutability in human cells selected for resistance to combination of UV and antipain" Mutation Res. 403. 215-222 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Sugita, K, Suzuki, N., Higuchi, Y., Kita, K., Suzuki, Y., and Lehmann, A: "Enhancement of XP-G mRNA expression by human interferon-β in Cockayne syndrome cells" Mutation Res. 408. 67-72 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Suzuki, N, Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S. and Suzuki, N.: "Mutagenicity of microcystin-LR in human RSa cells" International Journal of Molecular Medicine. 2. 109-112 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Higuchi, Y, Kita, K., Nakanishi, H., Wang, XL., Sugaya, S., Yanzawa, H., Yamamori, H., Sugita, K., Yamaura A. and Suzuki, N.: "Search for genes involved in UV-resistance in human cells by mRNA differential displey : increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance" BBRC. 248. 597-602 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Suzuki, N., Ishibashi, M., Kita, K., Wu, Y., Nomura, J., Takakubo, Y., Hiroshima, K., Genga, K., Ohwada, H. and Hayashi, Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells" Int.J.Cancer. 78. 550-555 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Wu, Y., Hiwasa, T, Isogai, E., Sonoda, T., Kita, K., Chen, Z., Sugaya, S., Yamamori, H., Takazawa, H. and suzuki, N.: "Activation of MAP Kinases by 5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line" Int.J.Oncol.13. 1241-1245 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Kikuno, K., Kita, K., Nomura, J., Hiwasa, T., Yonemitsu, H. and Suzuki, N.: "Search for genesresponsible for UV susceptibility of human cells : involvment of syndecan-1 in UV resistance" Biochem. Biophys. Res. Commun.253. 519-523 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tsuguhiko Tashiro, H.Yamamori, N.Hayashi, K.Furukawa, Y.Morishima, T.Sugiura, T.Itavashi, W.sano, Y.Toyoda, H.Nitta, J.Hirano, K.Nishiya and N.Nakajima, M.D.: "N-3 versus N-6 polyunsaturated fatty ascids (PUFA) in critical illness" Nutrition. 14. 551-553 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] N.Hayashi, T.Tashiro, H.Yamamori, K.Furukawa, Y.Morishima, T.Sugiura, T.Itabashi, W.Sano, Y.Toyoda, H.Nitta, J.Hirano, K.Nishiya and N.Nakajima: "Effect of intravenous n-3 and n-6 fat emulsion and delayed type hypersensitivity in burned rats receiving total parenteral nutrition" J Parent Ent Nutr. 22. 363-367 (1998)
Description
「研究成果報告書概要(和文)」より
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[Publications] Fuji, K., Suzuki, N., Kita, K., Ishijima, S,Sonoda, T., Dezawa, M., Sugita, K.and Niimi, H.: "Abnormal DNA synthesis activity induced by X-rays in nevoid basal cell carcinoma syndrome cells." Biochem.Biophys.Res.Commun.240. 269-272 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Isogai, E,Ishijima, S., Sonoda, T., Kita, K., Suzuki, H., Hasegawa, R., Yamamori, H., Takakubo, Y., and Suzuki, N.: "Protease activation following UV irradiation is linked to hypomutability in human cells selected for resistance to combination of UV and antipain" Mutation Res.403. 215-222 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sugita, K,Suzuki, N., Higuchi, Y., Kita, K., Suzuki, Y., and Lehmann, A.: "Enhancement of XP-G mRNA expression by human interferon-beta in Cockayne syndrome cells" Mutation Res.408. 67-72 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Suzuki, H,Watanabe, M., Wu, Y., Sugita, T., Kita, K., Sato, T., Wang, X., Tanzawa, H., Sekiya, S.and Suzuki, N.: "Mutagenicity of microcystin-LR in human RSa cells." International Journal of Molecular Medicine. 2. 109-112 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Higuchi, Y,Kita, K., Nakanishi, H., Wang, XL., Sugaya, S., Tanzawa, H., Yamamori, H., Sugita, K., Yamaura A.and Suzuki, N.: "Search for genes involved in UV-resistance in human cells by mRNA differential display : incrased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance." BBRC. 248. 597-602 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Suzuki, N., Ishibashi, M., Kita, K., Wu, Y., Nomura, J., Takakubo, Y., Hiroshima, K., Genga, K., Ohwada, H.and Hayashi, Y.: "Detection of Serum Factors Enhancing Cell Mutability From Lung Cancer Patients by Application of Hypermutable Human RS Cells." Int.J.Cancer. 78. 550-555 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Wu, Y., Hiwasa, T,Isogai, E., Sonoda, T., Kita, K., Chen, Z., Sugaya, S., Yamamori, H., Takazawa, H.and Suzuki, N: "Activation of MAP Kinases by-5-fluorouracil in a 5-fluorouracil-resistant variant human cell line derived from a KT breast cancer cell line." Int.J.Oncol.13. 1241-1245 (1998)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Kikuno, K., Kita, K., Nomura, J., Hiwasa, T., Yonemitsu, H.and Suzuki, H.: "Search for genes responsible for UV susceptibility of human cells : involvment of syndecan-1 in UV resistance." Biochem.Biophys.Res.Commun.253. 519-523 (1998)
Description
「研究成果報告書概要(欧文)」より
