1998 Fiscal Year Final Research Report Summary
Assessment of atherosclerosis obliterans by measuring NO concentration in muscle
| Project/Area Number |
09671212
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
SHIGEMATSU Hiroshi University of Tokyo, Surgery, Assistant Professor, 医学部・附属病院, 助教授 (40134556)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Yutaka University of Tokyo, Surgery, Assistant, 医学部・附属病院, 助手 (40311625)
KOMIYAMA Takashi University of Tokyo, Surgery, Assistant, 医学部・附属病院, 助手 (10292947)
HATAKEYAMA Takuya University of Tokyo, Surgery, Assistant, 医学部・附属病院, 助手 (60291324)
YASUHARA Hiroshi University of Tokyo, Surgery, Lecturer, 医学部・附属病院, 講師 (50251252)
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| Project Period (FY) |
1997 – 1998
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| Keywords | near-infrared spectroscopy / tissue oxygenation / NO / hemoglobin / atherosclerosis obliterans |
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| Research Abstract |
To measure NO concentration in muscle, we improved near-infrared spectroscopy and its computer analyzing system. We saturated hemoglobin medium with NO in several defined concentration, and measured NO hemoglobin absorbance as reference. In this modification, we could measure NO concentration in vitro.
In the experimental model, however, using New Zealand White rabbits, it was impossible to measure NO concentration in the muscle. But it was possible to assess the recovery of the oxygenation in the calf muscle after hindlimb ischemia caused by the ligation of the femoral artery. Therefore, we investigated that recovery process in the ischemic limbs in the rabbit experimental model.
The rabbits with ischemic limbs were divided into four groups. Group A ; which were fed on a standard diet, Group B ; which were fed on a high-cholesterol diet, Group C ; which were fed on a standard diet and treated with 5HT receptor antagonist (anti-platelet agent), Group D ; which were fed on a high-cholesterol diet and treated with 5HT receptor antagonist. There was no difference between Group A and Group C in ischemic recovery time (T1/2). T1/2 in Group D was shorter than that in Group B.There was statistically difference between Group B and Group D in T1/2. T1/2 in Group B was statistically longer than that in Group A.5HT of Group B in serum was higher than that in Group A.On the other hand, 5HT of Group B in whole blood was lower than that in Group A.
These data showed that 5HT receptor antagonist improved the muscle oxygenation after ischemia in this model. This study demonstrated that it was possible to measure tissue oxygenation in the animal model during exercise, as well as in the patients with atherosclerosis obliterans. The near-infrared spectroscopy analysis provide the valuable information to assess the drug effect not only in clinical setting but also in experimental model.
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Research Products
(14 results)
