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1998 Fiscal Year Final Research Report Summary

Development of a novel method for the selective expansion of human NK cells using immobilized lgG

Research Project

Project/Area Number 09671215
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionThe University of Tokyo

Principal Investigator

MIKAMO Shinsuke  The Institute of Medical Science, The University of Tokyo, Research Associate, 医科学研究所, 助手 (10107435)

Project Period (FY) 1997 – 1998
KeywordsNK cells / lgG / CD16 / CD25 / Adhesion / cell death / expansion / lL12
Research Abstract

Vujanovic and colleagues, described a procedure to expand antitumor effector cells from rat, and the technique was applied to human cancer therapy. The A-NK cells represented a small subset of PBMC, but expanded up to 1000-fold. Recently they tried to combine PBSCT and A-NK cell transfer, with better clinical outcome. We developed a new method to expand similar NK cells from resting NK cells from human PBMC, using immobilized IgG(ilgG).
Although NK cells were activated with ilgG, cell death was frequently observed. Overcontaminated Mo killed NK cells, A few % contamination showed dual effect, supporting NK cell growth and killing them, at the same time. .There might be exist activation induced cell death also.
The procedure consists of three steps. 1) Initial cell preparation and stimulation. 2) Activated NK cells became selectively adherent to the plastic and non-adherent T cells could be separated. 3) Adherent NK cells spontaneously detached from the plastic during the several hours of stimulation period and could be separated from contaminated Mo. The 3 steps offered differential treatments of the cultures.
Culture conditions, including co-existence of Mo and T cells, addition of IL2 and/or IL12, were tested. Then, a novel method was developed for activation without cell death.
NK cells from a healthy donor were activated by the method and a NK culture of high purity was obtained expanding 1000 fold. This method might bring forward a new strategy for adoptive immunotherapy.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Shinsuke Mikamo: "Development of a novel method for the selective expansion of human NK cells using immobilized IgG" Annual Report 1997,The Institute of Medical Science,The University of Tokyo. 191-192 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 美甘晋介,他: "局所サイトカイン療法への応用を考慮したNK細胞の選択的増殖法の開発" Biotherapy. 11(5). 634-636 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 美甘晋介,他: "固層化IgG刺激を用いたヒトNK細胞の選択的増殖法の開発" 第2回癌特異免疫療法研究会プログラム抄録集. 1-1 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Mikamo et al.: "Development of Selective NK Cell Expansion Method for Regional Cytokine Therapy" Biotherapy. 11. 634-636 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mikamo and M.Sekiguchi: "Developmrnt of Selective Activation Method for Human NK Cells Using Immobilized lgG." Proceedings of the Society for Tumor Specific Immuno-therapy. 2. 1-1 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Mikamo.: "Development of A Novel Method for The Selective Expansion of Human NK Cells Using Immobilized lgG." Annual Report 1997 Inst. Med. Sci. Univ. Tokyo.191-192 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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