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2000 Fiscal Year Final Research Report Summary

EXPLOITATION OF THE W/O/W TYPE EMULSIONS ENCAPSULATING BRM AGENTS AND INVESTIGATION IN THEIR ANTICANCER EFFECTS FOR LOCORESIONAL IMMUNOTHERAPIES

Research Project

Project/Area Number 09671221
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionGIFU UNIVERSITY

Principal Investigator

TAKAO Hiroshi  SCHOOL OF MEDICINE GIFU UNIVERSITY CANDIDATE, 医学部・附属病院, 講師 (50144035)

Project Period (FY) 1997 – 2000
Keywordsdrug delivery system / IL-2 / OK-432 / w / o emulsion / w / o / w emulsion / cマウス / colon26 / 細胞傷害活性 / NK活性
Research Abstract

【purpose】 We developed who type and w/o/w type emulsions as a drug delivery system. The emulsions which encapsulate BRM agents, were administrated for locoresional immunotherapies against cancer. Some trials for improvement of the emulsion was made for the anticancer effects.
【materials】 SD rats with liver metastases of MRMT-1 tumor cells and BALB/c mice with liver metastases of colon26 tumor cells, both tumor cells were inoculated into the portal vein of each animals. They were the subject of this study.
【methods】 Intrasplenic administration of the emulsion encapsulating BRM (IL-2, OK-432) immediately after inoculation of tumor cells was carried out. After several days liver cells and splenic cells were extracted measured cytotoxicity against both the autologous tumor cells (MRMT-1 and colon26) or NK sensitive cells (YAC-1)
【results】 The effects of w/o/w emulsion with BRM agents was better on cytotoxicity examination in the autologous tumor cells and in NK sensitive cells than those of w/o emulsion and the solution without BRM agents. And the effect was demonstrated immunopathologically. W/o/w emulsion was proved less side effects than that of w/o emulsion by additional experiments.
【conclusion】 From the results, w/o/w emulsion encapsulating BRM is suggested as a more effective and useful drug form especially for locoresional immunotherapy.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 安田邦彦: "BRM含有w/o/w型エマルジョンを用いた局所免疫療法に関する基礎的検討."Biotherapy. 10・3. 386-388 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田邦彦: "マウス肝転移モデルにおけるBRM含有エマルションの亢腫瘍効果に関する基礎的検討."Biotherapy. 11・3. 326-328 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安田邦彦: "OK-432,IL-2徐放性エマルジョンの肝転移抑制機序に関する検討."Biotherapy. 12・1. 83-86 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KUNIHIKO YASUDA: "Basic Studies on Locoregional Immunotherapy By W/O/W Emulsion Encapsulating BRM"Biotherapy. 10 : 3. 386-388 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUNIHIKO YASUDA: "Basic Studies on Antitumor Effect of W/O Emulsion Encapsulating BRM in a Tumor Bearing Mouse Model with Metastasis to Liver"Biotherapy. 11 : 3. 326-328 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUNIHIKO YASUDA: "Effect of Water-in-oil-Type Emulsion Encapsulating OK-432 and Interleukin-2 On Liver Metastasis of Cancer in Mice"Biotherapy. 12 : 1. 83-86 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MOTOHISA KATO: "Cancer Therapy by IL-2 of TNF"Biotherapy. 14 : 1. 5-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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