2000 Fiscal Year Final Research Report Summary
FHIT (fragile histidine triad) gene abnormality in lung cancer patients
| Project/Area Number |
09671258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | JIKEI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
AKIBA Tadashi Jikei University School of Medicine, Medicine, Lecturer, 医学部, 講師 (10192907)
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| Co-Investigator(Kenkyū-buntansha) |
ASAKURA Jyun Jikei University School of Medicine, Medicine, Assistant, 医学部, 助手 (80287193)
ODAKA Makoto Jikei University School of Medicine, Medicine, Assistant, 医学部, 助手 (20233554)
SHIOYA Hisashi Jikei University School of Medicine, Medicine, Assistant, 医学部, 助手 (70226108)
TAKAGI Masamichi Jikei University School of Medicine, Medicine, Assistant, 医学部, 助手 (70236199)
SATO Shuji Jikei University School of Medicine, Medicine, Lecturer, 医学部, 講師 (80205921)
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| Project Period (FY) |
1997 – 2000
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| Keywords | lung cancer / FHIT |
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| Research Abstract |
[Purpose] Investigate the significance of FHIT gene for lung cancer development from the correlation study of gene analysis using patients specimen and their clinical stage and prognosis.
[Methods] 1. Freeze the tumor obtained form the operation by liquid nitrogen.・Extract Poly A mRNA (use Fast Trzk Kit)・Extract of cDNA by Reversetranscriptase treatment. (use cDNA cycle kit)・Amplify FHIT gene by PCR.(use PCR primer specific to FHIT gene and cDNA as PCR primer) 2.・Extract RNA from free zed specimen (use SOGEN). Amplify FHIT gene a) Detection of abnormal band by EtBr staining after Electrophoresis of PCR products b) Identification of spricing error
3. Investigate the relation between the frequency of abnormality and the. observation of clinical stage, prognosis, and pathology.
[Results] 1. No abnormality of FHIT gene was found in five specimens. (Method 1) 2. The attempt of FHIT gene application was succeeded, but there were no abnormality bands in samples. (Method 2) 3. The samples are in the experimental stage. (Method 3)
[Discussion] The amplification of FHIT gene by PCR was failed (Method 1). The method of specimen collection (freeze, storage) may have caused the destruction of RNA and low yield of RNA is considered to be the bottom of the problem. The PCR amplified FHIT gene in Method 2 therefore the extraction of RNA by SOGEN seems to be a proper method. The experimental error (population problem of normal tissue and cancer cells in the specimens, dominant detection of noraml gene in trumor tissue) may lead to the failure of abnormal gene detection. It is early to conclude that FHIT gene doesn't participate in the development of lung cancer at this stage. It requires more specimens for the further study.
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