1997 Fiscal Year Annual Research Report

臨床乳癌検体からの腫瘍抗原特異的細胞障害性T細胞の検出とその標的抗原の同定

Research Project

Project/Area Number	09671269
Research Category	Grant-in-Aid for Scientific Research (C)
Research Institution	University of Occupational and Environmental Health, Japan
Principal Investigator	吉野一郎産業医科大学, 医学部, 講師 (40281547)
Co-Investigator(Kenkyū-buntansha)	市吉裕二産業医科大学, 医学部, 講師 (50264031)
Keywords	乳癌 / 細胞障害性T細胞 / 癌抗原 / HLA-A2
Research Abstract	【目的】乳癌局所リンパ節リンパ球より腫瘍特異的細胞障害性Tリンパ球を誘導し、その細胞障害性について検討する。【方法】乳癌患者2例より採取した局所リンパ節から濃度勾配遠心法にてリンパ球を採取し、48時間固層化抗CD3にて刺激培養後、低濃度IL-2下に2週間培養をおこなった。γ線にて不活化したPC-9(肺腺癌株)、またはMCF-7(乳癌細胞株)にて刺激培養を1週間に1回、合計3回行い,その細胞障害性を^<51>Cr release assay法、サイトカイン産生にて評価した。【結果、考察】PC-9で刺激誘導したCTLはPC-9に対してはclass Iを介した細胞障害性を示すが、MCF-7に対しては細胞障害性を示さなかった。またMCF-7にて刺激誘導したCTLはMCF-7に対してはclass Iを介した細胞障害性を示したが、PC-9に対しては障害性を示さなかった。これらよりPC-9、MCF-7上にそれぞれ異なる抗原が存在することが考えられ、CTLはそれを認識して細胞障害性を示すことが考えられた。【今後の展望】抗MCF-7を用いて腫瘍抗原の同定を行う。

Research Products
(6 results)

All Other

All Publications (6 results)

[Publications] Yoshino et al.: "The induction of cytotoxic T lymphocytes against HLA-A locus-matched long adenocarclnema in patlents with mon-small ceil lung cancer" Japanese Journal of Cancer Research. 88. 743-749 (1997)
[Publications] Yoshino et al.: "Postoperative plognosis in patiients with mon-small ceil lung cancer with synchronous intrapulmencry metastases" Annals of Thoracic Surgery. 64. 809-813 (1997)
[Publications] Takenoyama et al.: "Autologous cytotoxic T lymphocytes against human lung adenocarclnema recognize shared antigens presented byHLA-A24" Japanese Journal of Cancer Research. 89. 60-66 (1988)
[Publications] Yoshimatsu et al.: "Expression of melanoma antigen-encoding gene in human lung cancer" Journal of Surgical Ohcology. 67. 126-129 (1998)
[Publications] Yoshimatsu et al.: "Human lung cancer antigen presentation by tumor-assoolated macrophages to CD4^+ lymphocytes derived from regional lymph node" Annals of Cancer Research and Therapy. (in press).
[Publications] Hanagiri et al.: "Effect of interleukin-12 on the induction of cytotoxic T lymphocytes from the regional lymph node lymphocytes of patients with lung cancer" Japanese Journal of Cancer Research. (in press).

1997 Fiscal Year Annual Research Report

臨床乳癌検体からの腫瘍抗原特異的細胞障害性T細胞の検出とその標的抗原の同定

Principal Investigator

吉野 一郎 産業医科大学, 医学部, 講師 (40281547)

Research Products

[Publications] Yoshino et al.: "The induction of cytotoxic T lymphocytes against HLA-A locus-matched long adenocarclnema in patlents with mon-small ceil lung cancer" Japanese Journal of Cancer Research. 88. 743-749 (1997)

[Publications] Yoshino et al.: "Postoperative plognosis in patiients with mon-small ceil lung cancer with synchronous intrapulmencry metastases" Annals of Thoracic Surgery. 64. 809-813 (1997)

[Publications] Takenoyama et al.: "Autologous cytotoxic T lymphocytes against human lung adenocarclnema recognize shared antigens presented byHLA-A24" Japanese Journal of Cancer Research. 89. 60-66 (1988)

[Publications] Yoshimatsu et al.: "Expression of melanoma antigen-encoding gene in human lung cancer" Journal of Surgical Ohcology. 67. 126-129 (1998)

[Publications] Yoshimatsu et al.: "Human lung cancer antigen presentation by tumor-assoolated macrophages to CD4^+ lymphocytes derived from regional lymph node" Annals of Cancer Research and Therapy. (in press).

[Publications] Hanagiri et al.: "Effect of interleukin-12 on the induction of cytotoxic T lymphocytes from the regional lymph node lymphocytes of patients with lung cancer" Japanese Journal of Cancer Research. (in press).

吉野一郎産業医科大学, 医学部, 講師 (40281547)