1999 Fiscal Year Final Research Report Summary
p16 Expression Adenovirus Vector for Esophageal and Pancreas Cancer Therapy.
| Project/Area Number |
09671280
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Chiba University, School of Medicine |
|---|
Principal Investigator |
KOBAYASHI Susumu CHIBA UNIVERSITY SCHOOL OF MEDICINE SURGERY, ASSISTANTPROFESSOR, 医学部, 講師 (50234828)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIRASAWA Hiroshi CHIBA UNIVERSITY SCHOOL OF MEDICINE MICROBIOLOGY, PROFESSOR, 医学部, 教授 (00216194)
|
|---|
| Project Period (FY) |
1997 – 1999
|
| Keywords | P16 / Sdenovirus Vector / Pancreas Cancer / Gene Transfer / Esophageal Cancer |
|---|
| Research Abstract |
The prognoses of pancreas cancer patients have been miserable even after radical surgery, and the adjuvant therapy is necessary to improve the surgical results. P16ィイD1INK4aィエD1 (p16) is tight-binding and inhibitory protein for CDK4 to induce G1 arrest of cell cycle. P16 gene deletion is frequently identified in human pancreas cancer. The impaired gene function of p16 might be a major factor of the uncontrolled proliferation and malignancy of pancreas cancer cells. In this study, we investigated the effect of adenovirus p16 expression vector for pancreas cancer cell proliferation to clarify whether or not the vector might be a promising mode to assist the surgical therapy for pancreas cancer.
First, we constructed the adenovirus p16 expression vector (AdexCACSp16) by inserting p16 cDNA to a cassette cosmid containing a nearly full-length adenovirus type 5 genome with E1 and E3 deletions. Thereafter, we assessed the activity of AdexCACSp16 to induce p16 gene mRNA expression in pancreas cancer cell line, MIAPaCa-2 and to control the cell proliferation.
AdexCACSp16 induced the high level of p16 gene mRNA expression in MIAPaCa-2 cells with 1 hour contact to the cells. The cells proliferation was significantly suppressed by AdexCACSp16 compared with the control adenovirus group.
These data indicated that AdexCACSp16 has the potential to induce p16 gene expression and control pancreas cancer cell proliferation and that the adenovirus p16 expression vector AdexCACSp16 might be a possible method of gene therapy to improve the surgical therapeutic results for pancreas cancer.
|
