Study of Diagnostic Oncogene Analysis of Gastric Carcinoma

1998 Fiscal Year Final Research Report Summary

• Project/Area Number: 09671288
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Toyama Medical and Pharmaceutical University
• Principal Investigator: SHIMIZU Tetsuro Toyama Medical and Pharmaceutical University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (50260550)
• Co-Investigator(Kenkyū-buntansha): SAKAMOTO Takashi Toyama Medical and Pharmaceutical University, Faculty of Medicine, Associate Pro, 医学部, 助教授 (90162312)
• Project Period (FY): 1997 – 1998
• Keywords: tumor suppressor gene / gastric carcinoma / chromosome 14 / SSCP-PCR

Research Abstract

OBJECTIVE : The purpose of this study was to determine the precise frequency of loss of heterozygosity (LOH) and to define the correlation between some clinicopathological factors and LOH in gastric carcinoma. It might lead to some important informations useful for study of metastasis of gastric carcinoma. MATERIALS AND METHODS : Tumor and paired normal mucosal tissues were obtained from surgically resected specimens. Genomic DNA was extracted according to the standard proteinase K digestion and phenol-chloroform extraction method. Allelic deletions on 14q32 were assessed with a PCR (polymerase Chain Reaction)-SSCP (single-strand conformation polymorphism) method using following five microsatellite markers : D14S65, D14S68, D14S62, D14S267, and D14S250. RESULTS : The average rate of informative data was 42.7% in all cases of gastric carcinomas analyzed. The highest incidence (61%) of LOH was observed at the D14S65 locus within the five loci examined. As for the other loci, LOH was recognized in 61% at Dl 4S68, 52% at D14562, 41% at D14S267, and 26% at D14S250. There was a significant correlation between the extent of LOH and both of lymphatic invasion and lymph node metastasis of gastric carcinoma. CONCLUSIONS We firstly reported that allelic deletion at 14q32 associated with lymphatic spread of gastric carcinoma. These findings might not only become a foothold for molecular studies on lymphatic spread of cancer and also lead to precise diagnosis of advanced disease in gastric cancer.