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1998 Fiscal Year Final Research Report Summary

Antitumor effect of VEGF neutralizing antibody on gastrointestinal carcinoma.

Research Project

Project/Area Number 09671295
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

KONNO Hiroyuki  Hamamatsu Univ.School of Med., Dept.of Surg.II,Associate Professor, 医学部, 助教授 (00138033)

Co-Investigator(Kenkyū-buntansha) SUZUKI Hideo  Toagosei Co.Ltd., Tsukuba Research Laboratory, President of Bioscience Research, 所長
TANAKA Tatsuo  Hamamatsu Univ.School of Med., Dept.of Surg.II,Assistant Professor, 医学部, 助手 (90273185)
Project Period (FY) 1997 – 1998
KeywordsVEGF / Human Gastrointestinal Carcinoma / Metastasis / Neuralizing antibody
Research Abstract

Four human carcinoma xenografts, two human colon carcinomas and two gastric carcinomas, were transplanted orthotopically into nude mice : Northern blot analysis demonstrated the expression of VEGF mRNA in all 4 xenografts and VEGF protein was also demonstrated to be produced from all of them by ELISA.The VEGF neurtralizing antibody or same volume of saline was administered intraperitoneally on alternative days from day 10 after transplantation. With each of the four xenografts, administration of antibody significantly inhibited not only primary tumor growth but also macroscopic liver metastasis, although the growth rate varied among the 4 cancer xenografts. Body weight gain in each treated group was comparable with that in the control group. No toxicity of the antibody was observed. These results suggest that an anti-VEGF antibody can be effective against a wide variety of cancers and that VEGF may be a potent target for cancer therapy. The excellent therapeutic effect of netralizing antibody was also demonstrated against endocrine neoplasm derived from duodenum.
The mechanism of the effect of neutralizing antibody was also investigated. The inhibition of angiogenesis by neutralizing antibody which was shown quntitatively by immunohistochemistry followed by the apoptosis of tumor cells was mainly contributed to its inhibitory effect on tumor growth and metastasis.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kanai T., et al.: "Anti-tumor and anti-metastatic effects of human vascular endothelial growth factor neutralizing antibody on human colon and gastric carcinoma xenotransplanted orthotopically into nude mice." Int.J.Cancer. 77(6). 933-936 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konno H., et al.: "Antitumor effect of a neutralizing antibody to vascular endothelial growth factor on liver metastasis of endocrine neoplasm." Jpn.J.Cancer Res.89. 933-939 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiya K., et al.: "Antitumor effect and induction of apoptosis by monoclonal VEGF neutralizing antibody on the human gastric cancer." Jpn.J.Cancer Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之、他: "外科とサイトカイン-VEGF中和抗体の抗腫瘍効果-." 外科. 60(5). 542-545 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之: "抗血管新生による消化器癌治療." G.I.Reseach. 6(5). 384-388 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 馬場正三、他: "癌の血管新生抑制療法." Molecular Medicine. 35(10). 1262-1268 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之: "血管新生抑制物質とDormant therapy." 外科. 60(12). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之、他: "血管新生阻害剤による大腸癌肝転移治療." 日外会誌. 99(7). 441-445 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之、他: "増殖と血管新生因子." 医学のあゆみ. 184(12). 932 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 今野弘之、他: "血管新生阻害剤による消化器癌治療." Prog Drug Deliv System. VII. 49-59 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konno H,Tanaka T,Baba M,Kanai T,Matsumoto K,Kamiya K,Nakamura S,Baba S: "Quantitative analysis of vascular endothelial growth factor in colon cancer." Eur.Surg.Res. 30. 273-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kanai T,Konno H,Tanaka T,Baba M.Matsumoto K,Nakamura S,Yukita Y,Asano M,Suzuki H,Baba S.: "Anti-tumor and anti-metastatic effects of human vascular endothelial growth factor neutralizing antibody on human colon and gastric carcinoma xenotransplanted orthotopically into nude mice." Int.J.Cancer. 77 (6). 933-936 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Konno H,Arai T,Tanaka T,Baba M,Matsumoto K,Kanai T,Kamiya K,Nakamura S,Baba S,Nasito Y,Sugimura H,Yukita A,Asano M,Suzuki H.: "Antitumor effect of a neutralizing antibody to vascular endothelial growth factor on liver metastasis of endocrine neoplasm." Jpn.J.Cancer Res. 89. 933-939 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiya K,Konno H,Tanaka T,Baba M,Matsumoto K,Sakaguchi T,Yukita A,Asano M,Suzuki H,Arai T,, Nakamura S.: "Antitumor effect and induction of apoptosis by monoclonal VEGF neutralizing antibody on the human gastric cancer." Jpn.J.Cancer Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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