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1998 Fiscal Year Final Research Report Summary

Research on the prediction of hematogenous metastasis and recurrence of hepatocellular carcinoma, pancreatic carcinoma, gastric carcinoma and esophageal carcinoma. (Detection of circulating AFPmRNA,CEAmRNA and Cytokeratin mRNA)

Research Project

Project/Area Number 09671301
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SHIMADA Yutaka  Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical Basic Science, Assistant Professor, 医学研究科, 講師 (30216072)

Co-Investigator(Kenkyū-buntansha) IMAMURA Masayuki  Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical, 医学研究科, 教授 (00108995)
Project Period (FY) 1997 – 1998
KeywordsCirculating tumor cells in blood, / SCCmRNA, / CEAmRNA / AFPmRNA, / Cytokeratin20mRNA
Research Abstract

In this study, we established detection system (RT-nested PCR) of various gastrointestinal carcinoma cells in blood in the form of following mRNA.Alpha-fetoprotein(AFP) mRNA for hepatocellular carcinoma, carcinoembrionic carcinoma antigen (CEA) mRNA for pancreatic and gastric carcinoma, cytokeratin 20mRNA for colorectal carcinoma, and squamous cell carcinoma antigen (SCC) mRNA for esophageal carcinoma These systems could detect one to five cancer cells in 1ml of blood and magnetic cell absorption followed by immunohistochemistry clearly demonstrated the cells, which express CEA or Cytokeratin in peripheral blood. We also detected CEA mRNA in portal vein during operations in pancreatic carcinoma patients and SCC mRNA in peripheral blood during operations in patients with esophageal carcinoma. These facts show that carcinoma cells can be disseminated in blood during operations.
Detection of carcinoma related mRNAs in blood correlated with patients prognosis and recurrence. There were patients whose CEA mRNA or SCC mRNA was not detected after chemotherapy, however the protocols of drug administration remains controversial. Our detection systems of cancer cells in blood are useful for prediction of patients' prognosis and might have beneficial information for strategy of chemotherapy. Clinicopathological as well as molecular biological analysis of the patients with positive cancer cells in blood should be evaluated in the future.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Shimada Y: "Genetic Alterations in Patients with Esophageal Cancer with Short and Long Term Survival Rates after Curative Esophagectony" Ann Surg. 226. 162-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada Y: "Prognostic factors of oesophageal squamous cell carcinoma from the perspective of molecular biology" Br J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada Y: "Esophageal cancers Human Cell Culture" J. R. W. Masters and B. Palsson (eds) Kluwer Academic publishers., 34 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 嶋田 裕: "消化器病セミナー69 食道癌-診断と治療の最新の進歩" へるす出版, 21 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada Y.: "Genetic Alterations in Esophageal Cancer Patients with Short and Long Term Survival Rates after Curative Esophagectomy." Ann Surg. 226. 162-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada Y.: "Prognostic factors of oesophageal squamous cell carcinoma from the perspective of molecular biology." Br J Cancer. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada Y.: Esophageal cancers.Human Cell Culture J.R.W.Masters and B.Palsson (eds) Kluwer Academic publishers., 179-212 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada Y.: Genetic diagnosis and tumor marker of the esophageal cancer.Seminar of Gastroenterology 69 Esophageal cancer, Sugimachi K ed., Herusu Shuppan, 49-69 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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