1998 Fiscal Year Final Research Report Summary
Research on the prediction of hematogenous metastasis and recurrence of hepatocellular carcinoma, pancreatic carcinoma, gastric carcinoma and esophageal carcinoma. (Detection of circulating AFPmRNA,CEAmRNA and Cytokeratin mRNA)
| Project/Area Number |
09671301
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIMADA Yutaka Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical Basic Science, Assistant Professor, 医学研究科, 講師 (30216072)
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Masayuki Kyoto University, Graduate School of Medicine, Department of Surgery & Surgical, 医学研究科, 教授 (00108995)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Circulating tumor cells in blood, / SCCmRNA, / CEAmRNA / AFPmRNA, / Cytokeratin20mRNA |
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| Research Abstract |
In this study, we established detection system (RT-nested PCR) of various gastrointestinal carcinoma cells in blood in the form of following mRNA.Alpha-fetoprotein(AFP) mRNA for hepatocellular carcinoma, carcinoembrionic carcinoma antigen (CEA) mRNA for pancreatic and gastric carcinoma, cytokeratin 20mRNA for colorectal carcinoma, and squamous cell carcinoma antigen (SCC) mRNA for esophageal carcinoma These systems could detect one to five cancer cells in 1ml of blood and magnetic cell absorption followed by immunohistochemistry clearly demonstrated the cells, which express CEA or Cytokeratin in peripheral blood. We also detected CEA mRNA in portal vein during operations in pancreatic carcinoma patients and SCC mRNA in peripheral blood during operations in patients with esophageal carcinoma. These facts show that carcinoma cells can be disseminated in blood during operations.
Detection of carcinoma related mRNAs in blood correlated with patients prognosis and recurrence. There were patients whose CEA mRNA or SCC mRNA was not detected after chemotherapy, however the protocols of drug administration remains controversial. Our detection systems of cancer cells in blood are useful for prediction of patients' prognosis and might have beneficial information for strategy of chemotherapy. Clinicopathological as well as molecular biological analysis of the patients with positive cancer cells in blood should be evaluated in the future.
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