1998 Fiscal Year Final Research Report Summary
Novel cancer gene therapy using apoptosis-related gene
| Project/Area Number |
09671304
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMIZU Shigeomi Osaka University Medical School, assistant professor, 医学部, 助手 (70271020)
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| Co-Investigator(Kenkyū-buntansha) |
EGUCHI Yutaka Osaka University Medical School, associate professor, 医学部, 助教授 (20243206)
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| Project Period (FY) |
1997 – 1998
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| Keywords | caspase-3(CPP32 / Yama) / Bcl-2 / apoptosis |
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| Research Abstract |
Caspase-3 is a member of the cysteine protease family, which plays a crucial role in apoptosis.We applied the human caspase-3 gene as a novel form of anticancer gene therapy.Overexpression of human caspase-3 alone could not induce apoptosis of tumor cell lines, whereas apoptosis was markedly enhanced by the addition of VP-16.In an AH13O liver tumor model, transfection of human caspase-3, but not the empty vector, induced extensive apoptosis and reduced tumor volume, when applied together with VP-16.However, these effect was not observed with a Bcl-2 overexpressing tumor.In conclusion, caspase-3 gene transfection accompanied by an additional death stimulus may be useful anticancer gene therapy, except for Bcl-2 overexpressing tumors.
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