1998 Fiscal Year Final Research Report Summary
Experimental study of prevention of postoperative liver metastasis in pancreatic cancer
| Project/Area Number |
09671313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
NOSHIMA Shinji University Hospital, Yamaguchi University assistant, 医学部附属病院, 助手 (40243654)
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| Co-Investigator(Kenkyū-buntansha) |
ENOKI Tadahiko School of Medicine, Yamaguchi University assistant, 医学部, 助手
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| Project Period (FY) |
1997 – 1998
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| Keywords | pancreatic cancer / liver inetastasis / E-selectin / mouse / pancreatectomy / RT-PCR |
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| Research Abstract |
1. The changes of cytokines in the liver tissue after pancreatectomy - The effect of administration of mitomicin C into portal vein
Under intraperitoneal pentobarbital anesthesia, male balb/c mice were given distal pancreatectomy and splenectomy. The animals in the MMC group were administered mitomycin C (1mg/kg) into the portal vein soon after pancreatectomy, while the animals in the control group recieved no medication. The animals were killed 0 to 24 hours after surgery, and liver tissues were excised. After homogenization of the liver and centrifugation, tumor necrosis factor alpha, jnterleukin (IL) 1 beta , and IL 6 levels were determined by an enzyme-linked immunosorbent assay.
Tumor necrosis factor alpha and IL I beta did not change throughout the experiments in either groups ; however, IL 6 in the liver became significantly elevated from 0.680.27 and 0.43*0.20 pg/mg protein to 11.5*3.2 and 8.17*0.60 pg/mg protein, respevtively in the control and MMC groups, 30 minutes after surgery. This significant elevation of IL 6 persisted for 24 hours and 12 hours after surgery in the control and MMC groups, respectively.
2. Expression of the E-selectin (ELAM-1) gene in the liver tissue after pancreatectomy
E-selectin gene expressions in the liver were assayed by a reverse transcriptase-nested polymerase chain reaction. Expression of the E-selectin gene in the liver was not detected throughout the experimental period, while expression of the GAPDH gene was detected in every samples.
Thus, we concluded that pancreatectomy promoted IL 6 production in the liver and that rnitomycin C injection into the portal vein might reduce this elevation.
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