1998 Fiscal Year Final Research Report Summary
Growth factors and adhesion molecules in the progression of metastasizing capability of cancer cell lines.
| Project/Area Number |
09671332
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Jichi Medical School |
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Principal Investigator |
KAI Toshihiro Faculty of Medicine, Jichi Medical School Assistant Professor, 医学部, 講師 (40260830)
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| Co-Investigator(Kenkyū-buntansha) |
KUROKI Masatoshi Faculty of Medicine, Jichi Medical School Assistant Professor, 医学部, 助教授 (90215096)
HIKINO Hajime Faculty of Medicine, Jichi Medical School Assistant Professor, 医学部, 助手 (60296126)
TAKETAZU Fumitoshi Faculty of Medicine, Jichi Medical School Assistant Professor, 看護短期大学・教務部, 教授 (90254937)
MIYATA Michio Faculty of Medicine, Jichi Medical School Professor, 医学部, 教授 (90048976)
KAWAKAMI Masanobu Faculty of Medicine, Jichi Medical School Professor, 医学部, 教授 (40161286)
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| Project Period (FY) |
1997 – 1998
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| Keywords | ER-1 / ER-pP / TGF-β / TGF-β receptor / VEGF |
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| Research Abstract |
A rat breast cancer cell line ER-1, which cannot metastasize to the lung, forms lung metastatic lesions when it is cultured with TGF-beta 1. And three other cell lines (ER-pP1, -pP2, -pP3), derived from ER-1 by foreign substance reaction, make severe metastatic lesions to the lung. We studied about the growth factors and adhesion molecules in the difference of metastasizing capability of these cancer cell lines. ER-1 expressed the TGF-beta type I and type II receptors immunohistochemically, however other metasatable cell lines did not. And ER-1 cell count increased partially in soluble TGF-beta type II receptor treatment. These findings suggests that the TGF-beta may play a role of metastatic capability in these cell lines. All of these cell lines expressed VEGF responsibly with hypoxic stimulation in northern blotting. ER-1 treated with TGF-beta 1 for 30 days expressed increased level of TGF-beta 1 and VEGF in northern blotting. And there was not differential expression of E-cadherin and PCNA in both cells immunohistochemically. These results suggest that the difference of matastatic capability of these cell lines may concerned with TGF-beta and VEGF. We now continue to study the difference between these cell lines by molecular biological analysis.
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