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1999 Fiscal Year Final Research Report Summary

Telomerase activity in brain tumors.

Research Project

Project/Area Number 09671415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionGIFU UNIVERSITY

Principal Investigator

SAKAI Noboru  Gifu University, School of Medicine, Professor, 医学部, 教授 (10021487)

Co-Investigator(Kenkyū-buntansha) YOSHIMI Naoki  Gifu University, School of Medicine, Assistant Professor, 医学部, 助教授 (30166996)
IWAMA Toru  Gifu University, School of Medicine, Research Associate, 医学部附属病院, 助手 (20303498)
Project Period (FY) 1997 – 1999
KeywordsTelomerase / telomerase activity / brain tumor / TRAP assay / malignancy / differentiation
Research Abstract

Telomerase activity in brain tumors was analyzed. Telomerase activity was examined in surgically resected 41 brain tumors, A172 human glioblastoma cells, and C6 rat glioma cells by means of telomeric repeat amplification protocol (TRAP) assay. In the 41 tumors, the activity of telomerase was compared with histologic diagnosis, the MIB-1 proliferative cell index and patient's prognosis. In A 172 glioblastoma cells and C6 glioma cells, the changes in the telomerase activity of the cells were analyzed during differentiation induced by dibutyryl cyclic AMP, theophylline and retinoic acid. Telomerase activity in rat gliomas induced by ethylnitrosourea administration in pregnant rats was also examined.
Twelve of 20 glioblastomas, 2 of 2 oligodendrogliomas and 3 of 3 metastatic brain tumors demonstrated telomerase activity. However, anaplastic astrocytoma, low grade astrocytoma and meningioma exhibited no activity. Although no clear relations were confirmed in glioblastoma between telomerase activity and the MIB-1 proliferative cell index, the telomerase activity tended to correlate with patient's prognosis. After the cell differentiation, the telomerase activities of A 172 glioblastoma cells and C6 glioma cells were strongly decreased. There were no different telomerase activities among growth stages of rat gliomas induced by ethylnitrosourea.
These results suggest that telomerase activity may be an important marker of brain tumor malignancy and that functional inhibition of the activity may play a role during differentiation of glioma cells.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] K Nakatani, et al: "The significant role of telemerase activity in human brain tumors"Cancer. 80. 471-476 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J Shinoda, et al: "Clinical trial of external beam-radiotherapy combined with daily administration of low-dose cisplatin for supratentorial glioblastoma multiforme -A pilot study"J Neuro-Oncology. 35. 73-80 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K Nakatani, et al: "Alteration of telo, erase activity during differentiation of A172 human glioblastoma cells"Proceeding of 11th International Congress of Neurological Surgery. 1739-1743 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H Sakai, et al: "Molecular cloning of a cDNA encoding a serine protease homologous to complement C1s precursor from rat C6 glial cells and its expression during glial differentiation"Gene. 209. 87-94 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K Nakatani, et al: "The significance of the expression of tumor suppressor gene DCC in human gliomas"J Neuro-Oncology. 40. 237-242 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A Okumura, et al: "The characterization of human brain tumor using magnetization transfer technique in magnetic resonance imaging"Neuro Res. 21. 250-254 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H Sakai, S Nakashima, S Yoshimura, K Nakatani, K Takenaka, N Sakai, Y Nozawa: "Expression of CD44 variant isofomes and their relationship to cellular differentiation in human gliomas."Clinical Neurology and Neurosurgery. 99:Supple 1. s127 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, H Sakai, K Takenaka, J Shinoda, N Sakai: "Alteration of telomerase activity during differentiation of A172 human glioblastoma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Yoshimura, H Sakai, K Takenaka, K Nakatani, H Yamakawa, N Sakai, S Nakashima, Y Nozawa: "Intracellular signal transduction during ceramide-induced apoptosis in cultured glioma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Yoshimura, H Sakai, K Ohguchi, S Nakashima, Y Banno, Y Nishimura, N Sakai, Y Nozawa: "Changes in the activity and mRNA levels of phospholipase D during ceramide-induced apoptosis in rat c6 glial cells."Journal of Neurochemistry. 69. 713-720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, J Shinoda, N Sakai: "The significant role of telomerase activity in human brain tumors."Cancer. 80. 471-476 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J Shinoda, N Sakai, A Hara, T Ueda, H Sakai, K Nakatani: "Clinical trial of external beam-radiotherapy combined with daily administration of low-dose cisplatin for supratentorial glioblastoma multiforme -A pilot study."Journal of Neuro-Oncology. 35. 73-80 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Nakatani, N Yoshimi, H Mori, S Yoshimura, H Sakai, K Takenaka, J Shinoda, N Sakai: "Alteration of telo,erase activity during differentiation of A172 human glioblastoma cells."Proceeding of 11th International Congress of Neurological Surgery. 1739-1743 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Yoshimura, H Sakai, K Takenaka, K Nakatani, H Yamakawa, N Sakai, S Nakashima, Y Nozawa: "Intracellular signal transduction during ceramide-induced apoptosis in cultured glioma cells."Clinical Neurology and Neurosurgery. 99:Supple 1. s128 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Sakai, S Nakashima, S Yoshimura, Y Nishimura, N Sakai, Y Nozawa: "Molecular cloning of a cDNA encoding a serine protease homologous to complement Cls precursor from rat C6 glial cells and its expression during glial differentiation."Gene. 209. 87-94 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Murase, M Saio, K Takenaka, J Shinoda, Y Nishimura, N Sakai, T Takami: "Increased levels of CSF soluble CD27 in patients with primary central nervous system lymphoma."Cancer Letters. 132. 181-186 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K Nakatani, N Yoshimi, H Mori, H Sakai, J Shinoda, T Andoh, N Saka: "The significance of the expression of tumor suppressor gene DCC in human gliomas."J Neuro-Oncology. 40. 237-242 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A Okumura, K Takenaka, Y Nishimura, Y Asano, N Sakai, K Kuwata, S Era: "The characterization of human brain tumor using magnetization transfer technique in magnetic resonance imaging."Neurol Res. 21. 250-254 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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