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1999 Fiscal Year Final Research Report Summary

Neuroprotective effects of the hypothermia on permanent and transient cerebral ischemia

Research Project

Project/Area Number 09671444
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionSaitama Medical School

Principal Investigator

ASANO Takao  Saitama Medical School, Medicine, Professor, 医学部, 教授 (70090496)

Co-Investigator(Kenkyū-buntansha) MORIKAWA Eiharu  Saitama Medical School, Medicine, Assistant Professor, 医学部, 講師 (90251256)
MORIMOTO Tadashi  Saitama Medical School, Medicine, Associate Professor, 医学部, 講師 (20230154)
MATSUI Toru  Saitama Medical School, Medicine, Assistant Professor, 医学部, 助教授 (70199735)
TANI UCHI Tamiki  Saitarna Medical School, Medicine, Assistant Staff, 医学部, 助手 (30275888)
Project Period (FY) 1997 – 1999
Keywordspermanent focal cerebral ischemia / transient brain hypothermia / neuroprotection
Research Abstract

Whether brain hypothermia is established as a tool for the clinical treatment of the permanent focal cerebral isehemia remains to be yet. The first study aimed at comparing the effects of the 6 hour of selective brain hypothermia on final outcome of the experimental stroked animals.which were killed 6 (Experiment 1-1), 24 (Experiment l-2), 48 (Experiment 1-3) and 168 (Experiment 1-4) hours after occlusion ofleft middle cerebral artery.
A total of 116 Sprague-Dawley rats were divided into the above 4 groups, each of which included control animals (Group B)and sham-operated ones (Group C). They were endotracheally intubated and ventilated artificially. The proximal portion of the middle cerebral artery (MCAo) was electrically coagulated and then cut.
Our original methodology of the surface coil which perfused ice water enabled us to start selective brain hypothermia (SBH) immediately after MCAo during a period of 6 hours under general anesthesia (Group A). In the animals subjected to Group … More s B and C, the brain temperature was kept normal during the anesthesia. During the experiment, the brain temperature, the rectal temperature, blood pressure and blood gas were continually recorded. Each animal was extubated after the rewarming, and bred in the cultivating cage at room temperature until they were killed. At the end of experiments, the brain was perfused transcardially and removed. The brain slices were stained for measuring an infarction volume.
In the second experiment, our methodology was refined on, providing the less invasive method. The difference between these two methods is to keep anesthesia by mask and spontaneous respiration and only 4 hour of brain hypothermia immediately after MCAo in the refined one. The effects of SBH on infarct size at 168 hours of MCAo were compared among the SBH-, control and sham-operated groups.
The brain temperature of the SBH-group reached 32℃ within 20 minutes after the MCAo, and then stayed around 30 during the SBH. A complete rewarming needed only one hour. No abnormal finding was demonstrated in the systemic parameters. The infarction volume (mm3 mean ± standard deviation) was as follows. Experiment 1-1 (n=27) A (n=16)60 ±28, B (n=8) 130±6, C (n=3) 11±2; Experiment 1-2 (n=25) A (n=14) 84±25, B (n=6) 137±27, C (n=5) 4 ± 4; Experiment 1-3 (n=21) A (n=8) 33±11, B (n=8) 96±25, C(n=5) 7±7; Experiment 1-4 (n=l0) A (n=5) 41±20, B(n=3) 86±23, C(n=2) 6±7; Experiment 2 (n=33) A (n=11) 25±18, B (n=13) 100±41, C (n=9) 2±2
The present type of SBH is shown to significantly reduce the infarction volume up to 168 hours after the onset of ischemia, from the analysis of variance with the p-value of less than 0.05. The present results open up a new promising therapeutic strategy against the brain stroke. Less

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 谷口民樹、浅野孝雄、他: "ラット局所脳虚血モデルに対する脳局所冷却法の効果"第55回日本脳神経外科学会総会抄録集. 36(S1). 49 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "ラット局所永久脳虚血モデルに対する脳局所冷却法の効果"脳循環代謝. 8(1). 97 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taniguchi T, Asano T, et al: "Neuroprotective effects of selective brain hypothermia on permanent focal cerebra ischemia in rats"J. of Cerebral Blood Flow and Metabolism. 17(S1). S40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果の持続性"第9回日本脳循環代謝学会総会抄録集. 9(S1). 79 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果"日本脳低温療法研究会抄録集. 1(1). 9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果の持続性"脳循環代謝. 10(1). 76-77 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果"第10回日本脳循環代謝学会総会抄録集. 10(S1). 120 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果"脳循環代謝. 11(2). 182-183 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果"第11回日本脳循環代謝学会総会抄録集. 11(S1). 82 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷口民樹、浅野孝雄、他: "実験的局所永久脳虚血に対する脳冷却法の効果"脳循環代謝. 11(4). 438-439 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taniguchi T., Asano T., et al: "europrotective effects of selective brain hypothermia on focal IP-cerebral ischemia in rats."Medico Neurocirrugica. 36-S1. 49 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on permanent focal cerebral ischemia in rats."Japanese J Cereb Blood Flow Metabol. 8-1. 97 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on permanent focal cerebral ischemia in rats."J Cereb Blood Flow Metabol. 17-S1. S40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al.: "Prolonged neuroprotection after selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Cereb Blood Flow Metabol,. 9-S1. 79 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Brain Hypothermia. 1-1. 9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et: "Prolonged neuroprotection after selective brain hypothermia on experimental permanent focal cerebral ischemia"Japanese J Cereb Blood Flow Metabol,. 10-1. 76-77 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Cereb Blood Flow Metabol. 10-S1. 120 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Cereb Blood Flow Metabol. 11-2. 182-183 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Cereb Blood Flow Metabol. 11-S1. 82 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi T., Asano T., et al: "Neuroprotective effects of selective brain hypothermia on experimental permanent focal cerebral ischemia."Japanese J Cereb Blood Flow Metabol. 11-4. 438-439 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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