1998 Fiscal Year Final Research Report Summary
The efffect of irradiation on brain microvascular eudotherial cells
| Project/Area Number |
09671456
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KAWAMOTO Keiji Kansai Medical University, Faculty of medicine・Professor, 医学部, 教授 (70077741)
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| Co-Investigator(Kenkyū-buntansha) |
SUYAMA Takehiro Kansai Medical University, Faculty of medicine・Assistant, 医学部, 助手 (60288818)
TUCHIDA Takahiro Kansai Medical University, Faculty of medicine・Assistant Professor (lecturer), 医学部, 講師 (10181249)
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| Project Period (FY) |
1997 – 1998
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| Keywords | endotherial cell / gamma-ray / apoptosis / tPAI / irradiation |
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| Research Abstract |
Vascular endothellal cells are known to produce tissue plasminogen activator(t-PA) and plasminogen activator inhibitor 1(PAI-1). However, the release kinetics of these substances by brain microvascular endothelial cells (BMEC) is less well known. BMEC prepared from porcine brains using enzymatic digestion and percoll gradient separation was exposed to culture medium containing thrombiri (1.0IU/ml), histamine (10mumol/ml) and TNF-alpha(500, 1000, 2000U/ml). The supernatant obtained at respective time points was subjected to ELISA for t-PA, PAI-1 and t-PA-PAI- 1 complex. The value of these substances were significantly higher than those of the controls at respective time points. It also proved that t-PA and PAI-1 production by BMEC increases in a parallel fashion. The present study suggests that t-PA production by BMEC cultured under given stimulation increases, however, it is accompanied by concomittant PAl-1 production.
The brain inicrovascular endothereal cells were obtained from porci
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ne and were cultured by using our developed and simplified methods. These cultured cells were irradiated following with increasing doses and viability was examined with calorimetric assay methods. Proliferating cells showed the decrease of viability from the dose of SOGy. Semi confluent cells revealed the irradiation effct began 24hours later at 1000Gy, and 48hours later at 800Gy, but confluent cells were not effected even if over 1000Gy irradiation. Apoptotic cells showing the nuclear fragmentation were appeared at the proliferating and semi confluent stages. These data revealedthat Gamin ray was effective on the brain inicrovascular endotherial cells at proliferating stage, but these endotherial cells were strongly resistance at the non proliferating stage. Conclusion was introduced that new developed vessels were effected by irration such as the arteriovenous malfomation and brain desease of vascular tijmors, but normal brain vessels were not so injured following by hvdose of ceveral irradiation. Less
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