1998 Fiscal Year Final Research Report Summary
Biochemical and Biomechanical analysis of j-irradiated allogeneic tendon grafts
| Project/Area Number |
09671490
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka Prefectural College of Nursing (1998)
Osaka University (1997) |
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Principal Investigator |
KOUSEI Shino Division of Physical Therapy, Osaka Prefednal College of Health Science, Professor, 理学療法学科, 教授 (40135679)
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| Co-Investigator(Kenkyū-buntansha) |
SHIWADA Kozo Osaka Univ. Med. School, Assistant Professor, 医学部, 助手 (00216061)
NAKATA Ken Osaka Univ. Med. School, Assistant Professor, 医学部, 助手 (00283747)
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| Project Period (FY) |
1997 – 1998
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| Keywords | allograft / j-irradiation / biomechanics / biochemical study / freeze-drying / fresh-frozen |
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| Research Abstract |
We focused on the effect of radiation and freeze-drying of allogeneic tendons during the period of this Grant-in-Aid for Scientific Research.
1. Biochemical analysis of the irradiated, and freeze-dried allogeneic tendons after transplantation using rat animal model
We have established the rat allogeneic tendon/ligament transplantation model by substitution of the lateral half of the patellar tendon with allografts. These allografts had been treated radiation and freeze-drying. We found that radiation promoted turn-over of the collagen fibril after transplantation and Its effect was enhanced more in the freeze-dried tendons that in the fresh-frozen.
Biomechanical analysis of the irradated and freeze-dried allogeneic tendons.
We biomechanically analyzed the mechanical properties of the allogenic tendons using the same model as the biocheical analysis. Irradiation of the allogenic tendons decreased their tensile strength after transplantation. There was no statistically difference between the fresh-frozen group and in the freeze-dried one. The tensile strength of the grafts increased 6 weeks after transplantation in the both group.
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