1999 Fiscal Year Final Research Report Summary
Diaphragmatic fatigue during sepsis-Mechanisms and therapy
| Project/Area Number |
09671579
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Sapporo Medical University, School of Medicine |
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Principal Investigator |
WATANABE Hiroaki Sapporo Medical University, Asso. Pro, 医学部, 助教授 (20113764)
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| Co-Investigator(Kenkyū-buntansha) |
NARIMATSU Eichi Sapporo Medical University, Instructor, 医学部, 助手 (70295343)
SUMITA Shinzoh Sapporo Medical University, Asso. Pro., 麻酔科, 非常勤講師 (70187805)
FUJIMURA Naoyuki Sapporo Medical University, Training
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| Project Period (FY) |
1997 – 1999
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| Keywords | diaphragm / contractility / intra-abdominal sepsis / malondialdehyde / free radical / superoxide / dimethyl sulfoxide / catalase |
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| Research Abstract |
We investigated the alteration and mechanisms in diaphragmatic contractility in intra-abdominal sepsis in vitro. Intra-abdominal sepsis was produced using the cecal ligation and perforation technique (CLP). We assessed the diaphragmatic contractility by twitch characteristics and force-frequency curves in vitro. In the first study, we investigated the time course changes in diaphragmatic contractility after CLP. Diaphragmatic contractile dysfunction was began to develop 10hr after CLP and further reduction was observed 16hr after CLP. In the second study, we investigated the effects of PEG-SOD, PEG-CAT and DMSO on diaphragmatic contractility and MDA levels in septic peritonitis and the activities of two main antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx) in vitro. PEG-SOD, PEG-CAT and DMSO were administered intraperitoneally 30 min before and 12h after CLP. Diaphragmatic MDA levels were significantly elevated after CLP. PEG=SOD, PEG-CAT and DMSO significantly improved diaphragmatic contractility and prevented the elevation in diaphragmatic MDA levels after CLP. Diaphragmatic SOD activities were significantly increased after CLP. These results suggest that several types of oxygen-derived free radicals play a role in the reduction in diaphragmatic contractility after CLP.
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[Publications] FUJIMURA,N., SUMITA,S., NARIMATSU,E., NAKAYAMA,Y., SHITINOHE,Y., NAMIKI,A.: "Effects of Isoproterenol on Diaphragmatic Contractility in Septic Peritonitis."Am J Respir Crit Care Med. 161(2). 440-446 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NARIMATSU,E., NAKAYAMA,Y., SUMITA,S., IWASAKI,H., FUJIMURA,N., SATOH,K., NAMIKI,A.: "Sepsis attenuates the intensity of the neuromuscular effect of d-Tubocurarine and the antagonistic actions of neostigmine and edrophonium."Acta Anaesth Scand. 43. 196-201 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NARIMATSU,E., NAKAYAMA,Y., AIMONO,M., FUJIMURA,N., IWASAKI,H., NAMIKI,A.: "Milrinone, a Phosphodiesterase III inhibitor, antagonizes the neuromuscular blocking effect of a non-depolarizing muscle relaxant."Res Commun Mol Pathol Pharmacol. 104. 219-228 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NAKAYAMA,Y., NARIMATSU,E., SUMITA,S., FUJIMURA,N., SATOH,K., IWASAKI,H., NAMIKI,A.: "Propofol enhances a d-tubocurarine-induced twitch depression in septic rat diaphragm."Anesth Analg. 90(1). 80-84 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] NARIMATSU,E., NAKAYAMA,Y., SUMITA,S., IWASAKI,H., FUJIMURA,N., SATOH,K., NAMIKI,A.: "Sepsis attenuates the intensity of the neuromuscular blocking effect of d-tubocurarine and the antagonistic actions of neostigmine and edrophonium accompanying depression of muscle contractility of the diaphragm."Acta Anaesthesiol Scand. 43(2). 196-201 (1999)
Description
「研究成果報告書概要(欧文)」より
