1998 Fiscal Year Final Research Report Summary
Regulation of Pain via NMDA receptor-NO-cGMP pathway in the spinal cord
| Project/Area Number |
09671580
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TANAKA Yoshifumi Kyoto Prefectural University of Medicine, Department of Anesthesiology, Professor, 医学部, 教授 (50079935)
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| Co-Investigator(Kenkyū-buntansha) |
IBUKI Takae Kyoto Prefectural University of Medicine, Department of Anesthesiology, Associat, 医学部, 講師 (90232587)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Spinal Cord / Pain / Nitric Oxide / NMDA receptor |
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| Research Abstract |
The relationship between nitric oxide and glutamic acid in the dorsal horn of rat spinal cord were investigated in vivo and in vitro (slice preparation) using microsensors for nitric oxide and glutamic acid. As a result, although the microsensor for glutamic acid was proved not to have enough sensitivity in vivo, the sensor for nitric oxide showed biphasic increase of nitric oxide concentration in the dorsal horn of the spinal cord after for malin injection in the rat paw. The both responses were inhibited by pre-administration of 7-nitroindazole, which is reported to be a specific inhibitor of neuronal nitric oxide synthase (nNOS). In contrast, pre-administration of MK8O1 did not inhibit the first increase of nitric oxide after noxious stimuli, although it inhibited the second response. These reisults suggest that the first increase of nitric oxide was derived from dorsal root ganglion cells and the second response of nitric oxide was from dorsal horn neurons. The role of the nitric oxide in the spinal cord and the relationship between nitric oxide and glutamic acid are still under investigation.
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