1998 Fiscal Year Final Research Report Summary
Molecular Staging of Prostatic Cancer : Comparison of the Nasted Reverse Transcription Polymerase Chain Reaction Assays of Prostate Specific Antigen versus Prostate Specific Membrane Antigen as primers.
| Project/Area Number |
09671650
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyorin University School of Medicine |
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Principal Investigator |
HIGASHIHARA Eiji Kyorin University School of Medicine, Department of Urology, Professor, 医学部, 教授 (00092312)
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| Co-Investigator(Kenkyū-buntansha) |
森田 良治 杏林大学, 医学部, 非常勤講師
MURATA Akihiro Kyorin University School of Medicine, Department of Urology, Assistant, 医学部, 助手 (80291657)
OKEGAWA Takatsugu Kyorin University School of Medicine, Department of Urology, Assistant, 医学部, 助手 (70306679)
NUTAHARA Kikuo Kyorin University School of Medicine, Department of Urology, Assistant of Profes, 医学部, 助教授 (00143470)
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| Project Period (FY) |
1997 – 1998
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| Keywords | prostate cancer / prostate specific antigen / prostate specific membrane antigen / polymerase chain reaction |
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| Research Abstract |
The utility of nested reverse transcription polymerase chain reaction (RT-PCR) using prostate specific antigen (PSA) and prostate specific membrane antigen (PSM) primers for prostate cancer staging was examined.
1. The nested RT-PCR for the PSA and PSM primers was able to detect one LNCaP cell per 10^6 leukemia (K562) cells. None of control subjects were found positive by the nested RT-PCR.2. The nested RT-PCR using the PSM primers appears to predict the prostate cancer stage more accurately than do nested RT-PCR using PSA primers and the conventional clinical staging modalities. 3. The nested RT-PCR for PSM assay was the best preoperative predictor of biochemical recurrence among the parameters examined. 4. Nested RT-PCR for PSM assay may provide a more accurate estimation of the true incidence of micrometastatic prostate cancer cells in lymph node than pathological examination and was a better predictor of biochemical recurrence than nested RT-PCR for PSA and pathological examination.
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