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2000 Fiscal Year Final Research Report Summary

The significance of telomerase activation in cervical carcinogenesis

Research Project

Project/Area Number 09671696
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSaga Medical School

Principal Investigator

IWASAKA Tsuyoshi  Saga Med.Sch., Ob/Gyn, Professor, 医学部, 教授 (60117067)

Co-Investigator(Kenkyū-buntansha) UCHIYAMA Michiko  Saga Med.Sch., Ob/Gyn, Instructor, 医学部, 助手 (40274609)
YOKOYAMA Masatoshi  Saga Med.Sch., Ob/Gyn, Assistant professor, 医学部, 講師 (40230669)
Project Period (FY) 1997 – 2000
Keywordscervical carcinoma / telomerase / carcinogenesis / CIN / human papillomavirus / cervical screening
Research Abstract

1) Telomerase activity was detected in 88% of ovarian malignant tumors and in 100% of malignant tumors of the uterine corpus, the uterine cervix, and the vagina. While telomerase activity was not detected in the normal cervices and benign tumors, such as condyloma acuminatum and uterine leiomyoma.
2) Telomerase activity was found in HPV-immortalized and cigarette smoke condensate-transformed malignant cell lines established in a cervical carcinogenesis model and in cell lines derived from CINs and carcinomas, which suggests that telomerase activation is a relatively early event in cervical carcinogenesis.
3) Telomerase activity was detected in 8% of normal cervices, in 45% of patients with CIN (26% of patients with CIN I, 35% of CIN II, and 68% of CIN III, respectively), and in 97% of patients with invasive carcinoma. The results suggest that telomerase activation is an relatively early event in cervical carcinogenesis and correlates well with grade of cervical lesions.
4) Telomerase activity and HPV DNA sequences were examined in the exfoliated cervical cells from a general population of 245 women. Cytology revealed only one abnormal smear (1/245, 0.4%), in which a CIN I lesion positive for low-risk HPV DNA was found but the telomerase actiivity was negative. Telomerase activity was found in 16 exfoliated cell samples (16/245, 6.5%), high-risk HPV DNA was found in nine of these samples (9/16, 56%), and nine of the biopsy specimens that could be evaluated from patients testing positive for telomerase revealed CIN I lesions (9/11, 82%), which suggests that telomerase activity is often associated with high-risk HPV infection and that telomerase assay can help to detect occult cervical lesions.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Iwasaka T et al.: "Non detection of human papillomavirus DNA in cervical "Lancet. 348. 333-334 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng PS et al.: "Telomerase activity in gynecologic tumors."Gynecologic Oncology. 64. 171-175 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng PS et al.: "Telomerase activation in in vitro and in vivo cervical"Gynecologic Oncology. 66. 222-226 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka T et al.: "Prospective follow up of Japanese women with cervical"Int.J.Gynecol.Obstet.. 62. 269-277 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwasaka T et al.: "Telomerase activity in cervical neoplasia."Obstetrics and Gynecology. 91. 260-262 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng PS et al.: "Telomerase activity in Papanicolaou smear negative"Gynecologic Oncology. 77. 394-398 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩坂剛: "新女性医学大系、子宮頸部の悪性腫瘍"中山書店. 462 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zheng PS, Iwasaka T, Ouchida M, Fukuda K, Yokoyama M, Sugimori H: "Growth suppression of a cervical cancer cell line (TMCC-1) by the human wild-type p53 gene."Gynecol.Oncol.. 60. 245-250 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka T, Zheng PS, Ouchida M, Yamasaki H, Yokoyama M, Sugimori H: "Cytologic changes in two cervical carcinoma cell lines after transfection of the wild-type p53 gene."Acta Obstet.Gynecol.Scand.. 75. 797-803 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka T, Matsuo N, Yokoyama M, Sugimori H: "Non-detection of human papillomavirus DNA in cervical dysplasia and disease progression."Lancet. 348. 333-334 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zheng PS, Iwasaka T, Yamasaki F, Ouchida M, Yokoyama M, Nakao Y, Fukuda K, Matsuyama T, Sugimori H: "Telomerase activity in gynecologic tumors."Gynecol.Oncol.. 64. 171-175 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zheng PS, Iwasaka T, Yokoyama M, Nakao Y, Pater A, Sugimori H: "Telomerase activation in in vitro and in vivo cervical carcinogenesis."Gynecol.Oncol.. 66. 222-226 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama M, Iwasaka T, Matsuo N, Hachisuga T, Mori M, Sugimori H: "Correlation between human papillomavirus positivity and p53 gene overexpression in adenocarcinoma of the uterine cervix."Gynecol Oncol. 65. 23-29 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka T, Matsuo N, Yokoyama M, Uchiyama M, Fukuda K, Sugimori H: "Prospective follow-up of Japanese women with cervical intraepithelial neoplasia and various human papillomavirus types."Int.J.Gynecol.Obstet.. 62. 269-277 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwasaka T, Zheng PS, Yokoyama M, Fukuda K, Nakao Y, Sugimori H: "Telomerase activation in cervical neoplasia."Obstet.Gynecol. 91. 260-262 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zheng PS, Iwasaka T, Zhang ZM, Pater A, Sugimori H: "Telomerase activity in Papanicolaou smear-nagative exfoliated cervical cells and its association with lesions and oncogenic human papillomaviruses."Gynecol.Oncol.. 77. 394-398 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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