1999 Fiscal Year Final Research Report Summary
EXLPLOITATION OF NEW DIAGNOSTIC PROCEDURES OF HUMAN ENDOMETRIAL CANCERS IN RELATION TO CYTOGENETIC MECHANISMS OF MULTI-STEP CARCINOGENESIS ELUCIDATED WITH CGH
| Project/Area Number |
09671726
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | JAPANESE FOUNDATION OF CANCER RESEARCH |
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Principal Investigator |
HIRAI Yasuo CANCER INSTITUTE, JAPANESE FOUNDATION OF CANCER RESEARCH, RESEARCH FELLOW, 癌研究所・細胞生物部, 研究員 (00260076)
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| Project Period (FY) |
1997 – 1999
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| Keywords | ENDOMETRIAL CARCINOMA / TGFβRII / BAX / β-catenin / PTEN / MICROSATELLITE INSTABILITY / COMPARATIVE GENOMIC INSTABILITY / MULTISTEP CARCINOGENESIS |
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| Research Abstract |
We analyzed the mutational status of the TGFβRII, BAX, and PTEN/MMAC1 genes as well as microsatellite instability (MI) in 29 consecutive cases of endometrial carcinoma operated on at our Institute. To examine copy number loss at the chromosomal regions bearing these genes, we used comparative genomic hybridization (CGH) analysis. CGH analysis may provides a genome-wide overview about tumor-associated genomic imbalances. Among nine tumors that showed the MI+ phenotype, four (44%) demonstrated a significant mutation with a definite amino acid change in PTEN/MMAC1 gene. CGH analysis demonstrated that all the four tumors (100%) showed chromosomal copy number loss around the locus of this gene, whereas four (57%) of seven tumors with PTEN/MMAC1 mutations showed chromosomal loss or double mutations in MI- carcinomas. The role of TGFβRII and BAX genes is limited as a target gene of MI+ phenotype in endometrial cancer, because several mutations of these genes were detected but a chromosomal loss was demonstrated by CGH in only one tumor in MI+ endometrial cancers with mutation. According to CGH results, chromosomal imbalances were demonstrated at 1q, 9q, 2q, 12q. Frequent abnormal cytoplasmic/ nuclear accumulation of β-catenin was observed in this endometrial series.
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[Publications] H Yoshikawa, C Nagata, K Noda, S Nozawa, A Yajima, S Sekiya, H Sugimori, Y Hirai, K Kanazawa, M Sugase, H Shimizu, T Kawana: "Human papillomavirus infection and other risk factors for cervical intraepithelial neoplasia in Japan"British Journal of Cancer. 80. 621-624 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] C Nagata, H Shimizu, H Yoshikawa, K Noda, S Nozawa, A Yajima, S Sekiya, H Sugimori, Y Hirai, K Kanazawa, M Sugase, T Kawana: "Serum carotenoids and vitamins and risk of cervical dysplasia from a case-control study in Japan"British Journal of Cancer. 81. 1234-1237 (1999)
Description
「研究成果報告書概要(欧文)」より
